TRP Channels

Methods and Protocols

Antonio Ferrer-Montiel, Tim Hucho (Herausgeber)

Buch | Hardcover

238 Seiten

2019 | 2019 ed.
Humana Press Inc. (Verlag)
9781493994458 (ISBN)

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This book aims to provide a guide to researchers on how to work with Transient Receptor Potential (TRP) channels, describing current methodologies and protocols, and highlighting the challenges ahead that will require the development of new technologies. Articles cover a wide range of techniques, from structure to function, concluding with how to best to evaluate some of the in vivo function of TRP channels, particularly in regard to their involvement in hyperalgesia and allodynia. Also, the collection describes important and inspiring available tools that are currently being used to investigate the activity of these channels in vitro and in vivo in terms of their underlying protein structure, segueing into dealing with the generation of pharmacological tools to explore their in vivo activity and their involvement in cellular signaling pathways, which may be developed into drugs to treat diseases caused by channel dysfunction or hyperactivity. Written for the highly successful Methods in Molecular Biology series, chapters include the necessary detail and implementation advice to ensure successful results in the lab.
Authoritative and practical, TRP Channels: Methods and Protocols is a useful set of methodologies for scientists seeking to better understand this vital family of channels.

Molecular Evolution Bioinformatics towards Structural Biology of TRPV1-4 Channels.- Expression, Purification, and Crystallization of the Transient Receptor Potential Channel TRPV6.- Cryo-Electron Microscopy of TRP Channels.- Mass Spectrometry-Based Analysis of TRPP2 Phosphorylation.- Combining Structural Data with Computational Methodologies to Investigate Structure-Function Relationships in TRP Channels.- Characterization of TRPC Channels in a Heterologous System Using Calcium Imaging and the Patch-Clamp Technique.- Fluorescence-Based Functional Assays for Ca2+ Permeable ThermoTRP Channels.- High Content Imaging of Immunofluorescently-Labeled TRPV1-Positive Sensory Neurons.- Patch-Clamp Combined with Fast Temperature Jumps to Study Thermal TRP Channels.- TRP Channels Reconstitution in Lipid Bilayers.- Methods for Investigating TRP Channel Gating.- In Silico Approaches for TRP Channel Modulation.- Drug Discovery for Soft Drugs on TRPV1 and TRPM8 Channels Using the Passerini Reaction.- In Vivo Methods to Study ThermoTRP Channels in Rodents.

Erscheinungsdatum	17.05.2019
Reihe/Serie	Methods in Molecular Biology ; 1987
Zusatzinfo	58 Illustrations, color; 11 Illustrations, black and white; X, 238 p. 69 illus., 58 illus. in color. With online files/update.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Pharmazie
	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Chemie ► Technische Chemie
	Technik
Schlagworte	cellular signaling pathways • Channel dysfunction • Non-selective cationic channels • pharmacology • Protein Structure • therapeutics • Transient receptor potential channels
ISBN-13	9781493994458 / 9781493994458
Zustand	Neuware