Immunological Tolerance

Methods and Protocols

Buch | Hardcover

240 Seiten

2019 | 2019 ed.
Humana Press Inc. (Verlag)
978-1-4939-8936-2 (ISBN)

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This edition provides a detailed, up-to-date overview of methods used in the field of immune tolerance. Chapters guide readers through tolerogenic cell types, isolating tolerogenic cell populations for study and therapeutic utility, multiple methods to study the mechanisms underpinning tolerance, methods to induce tolerance through thymus progenitors, and methods to assess the breakdown of immune tolerance in specific pathological conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Immunological Tolerance: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Isolation and Characterisation of Human Adipose-derived Stem Cells.- Isolation of Murine Hematopoietic Stem Cells.- Generation of Antigen-specific T cells from Human Induced Pluripotent Stem Cells.- Isolation of Human Regulatory T Lymphocytes by Fluorescence-activated Cell Sorting .- Identification and isolation of Regulatory B cells in Mouse and Human.- An innovative Standard Operation Procedure for Isolating GMP-grade CD4+CD25+ T Cells from non-mobilized leukapheresis.- Measuring CTLA-4-dependent Suppressive Function in Regulatory T Cells.- Alloanergization Method for Inducing Allospecific Hyporesponsiveness in PBMC Exposed to Allostimulation in vitro in the Context of Costimulatory Molecule Blockade.- In Vitro Suppression of CD4+ T Cell Responses by Murine and Human Myeloid-derived Suppressor Cells.- Disassembling and Re-aggregating the Thymus: The Plus and Minus of Current Assays.- Isolation of Highly Viable Thymic Epithelial Cells for use in In Vitro and In Vivo Experiments.- Immune tolerance and rejection in organ transplantation.- Method of Generating Tolerogenic Maturation-resistant Dendritic Cells and Testing for their Immune-regulatory Functions In Vivo in the Context of Transplantation.- Luminex Coupled-EliFACS: A Multiparametric Method to Enumerate and Functionally Characterize Autoreactive T Cells in Human Peripheral Blood.- Clinical and Functional Evaluation of Ocular Inflammatory Disease Using the Model of Experimental.- Methods of Isolation and Analysis of TREG Immune Infiltrates from Injured and Dystrophic Skeletal Muscle.

Erscheinungsdatum	05.02.2019
Reihe/Serie	Methods in Molecular Biology ; 1899
Zusatzinfo	36 Illustrations, color; 7 Illustrations, black and white; XI, 240 p. 43 illus., 36 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
Themenwelt	Studium ► Querschnittsbereiche ► Infektiologie / Immunologie
Schlagworte	B regulatory populations • self-renewing cell • Stem Cells • thymus progenitors • tolerogenic cell • tolerogenic lymphocytic cells
ISBN-10	1-4939-8936-7 / 1493989367
ISBN-13	978-1-4939-8936-2 / 9781493989362
Zustand	Neuware