Promoter Associated RNA

Methods and Protocols

Buch | Softcover

287 Seiten

2018 | Softcover reprint of the original 1st ed. 2017
Humana Press Inc. (Verlag)
978-1-4939-8281-3 (ISBN)

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This volume is divided in four sections; covering genome wide approaches, techniques for characterize of paRNA structural features are described, selecting pa-RNA, and paRNA therapeutic potential. Chapters describe how siRNAsdirected against paRNAs can be applied in vivo to modulate transcription of important genes controlled by paRNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Promoter Associated RNA: Methods and Protocols aims to demonstrate paRNAs as new class of regulatory molecules, to further investigate and value as tools for fine transcriptional tuning.

ChIP-seq for the Identification of Functional Elements in the Human Genome.- Identification of Candidate Functional Elements in the Genome from ChIP-seq Data.- GRO-seq, A Tool for Identification of Transcripts Regulating Gene Expression.-NanoCAGE: A Method for the Analysis of Coding and Non-coding 5’-capped Transcriptomes.- Deep Cap Analysis of Gene Expression (CAGE): Genome-Wide Identification of Promoters, Quantification of their Activity Andtranscriptional Network Inference.- Deep-RACE: Comprehensive Search for Novel ncRNAs Associated to a Specific Locus.- In silico Prediction of RNA Secondary Structure.- Computational Prediction of RNA-Protein Interactions.- Isolation of Nuclear RNA-Associated Protein Complexes.- Identification of Long Non-Coding RNAs Associated to Human Disease Susceptibility.- Targeting Promoter-associated RNAs by siRNAs.- RNA-FISH to Study Regulatory RNA at the Site of Transcription.- Detection and Characterization of R Loop Structures.- Induction of Transcriptional Gene Silencing by Expression of shRNA Directed to c-Myc P2 Promoter in Hepatocellular Carcinoma by Tissue Specific Virosomal Delivery.- Targeting Promoter Associated Non-coding RNA In Vivo.- Manipulation of Promoter-Associated Noncoding RNAs in Mouse Early Embryos for Controlling Sequence-Specific Epigenetic Status.

Erscheinungsdatum	25.08.2018
Reihe/Serie	Methods in Molecular Biology ; 1543
Zusatzinfo	182 Illustrations, color; 101 Illustrations, black and white; X, 287 p. 283 illus., 182 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
Themenwelt	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
Schlagworte	noncoding RNA • noncoding transcriptome • paRNA • promoter-associated RNA • R-loop structures • RNA binding proteins
ISBN-10	1-4939-8281-8 / 1493982818
ISBN-13	978-1-4939-8281-3 / 9781493982813
Zustand	Neuware