Performance capability evaluation of drug-protein binding studies

Pharmacokinetic research in biological fluids

Oliver Wollner (Autor)

Buch | Softcover

72 Seiten

2017
AV Akademikerverlag
978-620-2-20588-7 (ISBN)

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The analysis of drug binding by endogenous proteins plays a major role in pharmaceutical drug development. Current binding studies are mainly made with plasma as it is presumed that unbound drugs can move freely between tissues. As this hypothesis has its limits it would be necessary to take the interstitial fluid into account as well. The interstitium is the area where most of the drugs mediate their effect. This fluid, however, is difficult to access due to the narrow space between tissue cells, but modern sampling approaches have shown promising results. It is unclear whether binding essays can also function in fluids with a low-protein content such as the interstitium. In this thesis drug protein binding essays were evaluated with several biological fluids to highlight possible differences in drug binding capacities between tissues. Therefore, several techniques were investigated and compared. Analysis of drug concentrations were performed via LC-MS.

Oliver Wollner attained his Bachelor degree in Biochemistry at the University of Würzburg and wrote his thesis at the Cancer Center at the University Hospital. Then he graduated with a Master of Science degree in Biotechnology at the Graz University of Technology with distinction. He wrote his thesis about pharmacokinetics at Joanneum Research.

Erscheinungsdatum	23.09.2022
Sprache	englisch
Maße	150 x 220 mm
Gewicht	124 g
Themenwelt	Medizin / Pharmazie ► Pharmazie
Schlagworte	Albumin • alpha 1-acid glycoprotein • drug-protein binding • free-drug hypothesis • HPLC-MS • low-protein fluids • pH influence • rapid equilibrium dialysis • solid-phase microextraction • temperature influence • Ultrafiltration
ISBN-10	620-2-20588-1 / 6202205881
ISBN-13	978-620-2-20588-7 / 9786202205887
Zustand	Neuware