Methods in Virology (eBook)

Volume VIII

Hilary Koprowski, Karl Maramorosch (Herausgeber)

eBook Download: PDF

2014 | 1. Auflage
414 Seiten
Elsevier Science (Verlag)
978-1-4832-5901-7 (ISBN)

Infos

Methods in Virology, Volume VIII focuses on the methods used in virology, including microscopy, hybridization, viruses, and fingerprint analysis. The selection first offers information on the hybridization of viral nucleic acids; applications of oligonucleotide fingerprinting to the identification of viruses; and immunosorbent electron microscopy in plant virus studies. Discussions focus on the detection of double-stranded RNA, principles and mechanisms of fingerprint analysis, preparation of labeled nucleic acid probes, and basic methods of nucleic acid hybridization. The text then elaborates on quantitative transmission electron microscopy for the determination of mass-molecular weight of viruses and use of thin sectioning for visualization and identification of plant viruses. Topics include technical procedures for processing plant tissues, cytological modifications of diagnostic value, procedure and treatment of data to obtain the average mass of virus particles, and applications in virology. The book takes a look at the detection of genome-linked proteins of plant and animal viruses; methods for assay, purification, and characterization of prions; and the use of mosquitoes to detect and propagate viruses. The selection is a valuable source of information for researchers interested in the methods employed in virology.

Inhalt

Front Cover 1
Methods in Virology 4
Copyright Page 5
Table of Contents 6
Contributors 10
Preface 12
Contents of Previous Volumes 14
Chapter 1. Hybridization of Viral Nucleic Acids: Newer Methods on Solid Media and in Solution 20
I. Introduction 20
II. Purification of Nucleic Acid 22
III. Preparation of Labeled Nucleic Acid Probes 26
IV. Basic Methods of Nucleic Acid Hybridization 29
V. Southern Blot Hybridization 42
VI. Northern Blots 56
REFERENCES 57
Chapter 2. Applications of Oligonucleotide Fingerprinting to the Identification of Viruses 60
I. Introduction 60
II. Principles and Mechanisms of Fingerprint Analysis 62
III. Experimental Procedures 68
IV. Applications 91
V. Summary and Conclusions 98
ACKNOWLEDGMENTS 99
REFERENCES 99
Chapter 3. Immunosorbent Electron Microscopy in Plant Virus Studies 104
I. Introduction 104
II. Parameters of the Method 106
III. Varying Success with ISEM 115
IV. Applications 115
V. Detection of Double-Stranded RNA 117
VI. Preparation of Grids for Later Use 117
VII. Problems and Prospects for the Future 118
REFERENCES 119
Chapter 4. Quantitative Transmission Electron Microscopy for the Determination of Mass–Molecular Weight of Viruses 122
I. Introduction 122
II. Theory 124
III. Procedure and Treatment of Data to Obtain the Average Mass of Virus Particles 136
IV. Applications in Virology 142
V. Discussion 152
VI. Summary 157
ACKNOWLEDGMENTS 158
REFERENCES 158
Chapter 5. Use of Thin Sectioning for Visualization and Identification of Plant Viruses 162
I. Introduction 162
II. Technical Procedures for Processing Plant Tissues 163
III. Cytological Modifications of Diagnostic Value 197
ACKNOWLEDGMENTS 235
REFERENCES 235
Chapter 6. Purification and Immunological Analyses of Plant Viral Inclusion Bodies 244
I. Introduction 244
II. Inclusion Purification 245
III. Serological Analyses 273
IV. Discussion 296
ACKNOWLEDGMENTS 297
REFERENCES 297
Chapter 7. Use of Mosquitoes to Detect and Propagate Viruses 300
I. Rationale for Use of the Technique 300
II. Technical Considerations 301
III. Possible Limitations and Pitfalls 309
REFERENCES 311
Chapter 8. Prions: Methods for Assay, Purification, and Characterization 312
I. Introduction 313
II. Assays for Scrapie Prions 314
III. Histopathology 321
IV. Purification 323
V. Identification and Detection of PrP 335
VI. Electron Microscopy 345
VII. Polarization Microscopy 351
VIII. Biocontainment and Decontamination Procedures 353
ACKNOWLEDGMENTS 362
REFERENCES 362
Chapter 9. Detection of Genome-Linked Proteins of Plant and Animal Viruses 366
I. Introduction 366
II. Detection of a Genome-Linked Protein (VPg) 367
III. Characterization of Genome-Linked Proteins and Adjacent Nucleotide Sequences 379
IV. Biosynthesis of Genome-Linked Proteins 387
V. Possible Functions of Genome-Linked Proteins 389
REFERENCES 395
Index 400

Produkt-Details

• Erscheint lt. Verlag: 28.6.2014
• Sprache: englisch
• Themenwelt: Medizin / Pharmazie ► Medizinische Fachgebiete
• Themenwelt: Studium ► Querschnittsbereiche ► Infektiologie / Immunologie
• Themenwelt: Naturwissenschaften ► Biologie ► Evolution
• Themenwelt: Technik
• ISBN-10: 1-4832-5901-3 / 1483259013
• ISBN-13: 978-1-4832-5901-7 / 9781483259017