Pathobiology of the Endothelial Cell (eBook)

Hymie L. Nossel, Henry J. Vogel (Herausgeber)

eBook Download: PDF

2013 | 1. Auflage
532 Seiten
Elsevier Science (Verlag)
978-1-4832-1896-0 (ISBN)

Infos

Pathobiology of the Endothelial Cell presents the proceedings of the symposium on the 'Pathobiology of the Endothelial Cell' held at Arden House, on the Harriman Campus of Columbia University on June 5-7, 1981. The book discusses the endothelial growth regulation; the stimulation of vascular cell growth by macrophage products; and the control of proliferation and differentiation of endothelial cells. The text also describes vessel wall growth control; the implications of angiogenesis in vitro for tumor biology; and the interactions and activation of coagulation proteases. Platelet adhesion and fibrinogen proteolysis; endothelial protein synthesis; prostaglandins, and endothelial cell-cell interactions are also considered. The book further tackles topics on vascular pathobiology. Biologists, pathologists, biochemists, hematologists, oncologists, immunologists, and microbiologists will find the text invaluable.

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Pathobiology of the Endothelial Cell presents the proceedings of the symposium on the "e;Pathobiology of the Endothelial Cell"e; held at Arden House, on the Harriman Campus of Columbia University on June 5-7, 1981. The book discusses the endothelial growth regulation; the stimulation of vascular cell growth by macrophage products; and the control of proliferation and differentiation of endothelial cells. The text also describes vessel wall growth control; the implications of angiogenesis in vitro for tumor biology; and the interactions and activation of coagulation proteases. Platelet adhesion and fibrinogen proteolysis; endothelial protein synthesis; prostaglandins, and endothelial cell-cell interactions are also considered. The book further tackles topics on vascular pathobiology. Biologists, pathologists, biochemists, hematologists, oncologists, immunologists, and microbiologists will find the text invaluable.

Inhalt

Front Cover 1
Pathobiology of the Endothelial Cell 4
Copyright Page 5
Table of Contents 6
List of Participants 14
Preface 22
Opening Address: Differentiated Microdomains in the Vascular Endothelium 24
Structural Substrate of Capillary Permeability 24
Surface Chemistry of the Vascular Endothelium—Differentiated Microdomains 26
Digestive Enzyme Tests 29
Microdomains and Capillary Permeability 31
Microdomains and Membrane Specificity 33
References 34
Part I: Endothelial Growth Regulation 38
Chapter 1. Stimulation of Vascular Cell Growth by Macrophage Products 40
Introduction 40
Macrophage-Induced Neovascularization: In Vivo Studies 41
Stimulation of Cell Growth in Vitro by Macrophage Products 44
Modulation of Macrophage-Derived Growth Factor Production 48
Sources of Macrophage-Derived Growth Factor 49
Properties of Macrophage-Derived Growth Factor 49
Biological Significance of Macrophage-Derived Growth Factor 51
Summary 52
References 53
Chapter 2. The Control of Proliferation and Differentiation of Endothelial Cells 56
Introduction 56
The Cornea: Structure and Development 56
Effects of Growth Factors on the Proliferation and Differentiation of Corneal Endothelial Cells in Vitro 58
Involvement of the Basal Lamina Produced by Corneal Endothelial Cells in the Control of Cell Proliferation 67
The Use of Corneal Endothelial Cell Cultures in Studies of Their Differentiated Properties 73
The Use of the Basal Lamina Produced by Cultured Corneal Endothelial Cells for Studies of the Control of Proliferation of Malignant Versus Normal Cells 80
Effect of Basal Lamina on the Attachment, Migration, Proliferation, and Differentiation of Nerve Cells 83
Conclusion 92
References 93
Chapter 3. Vessel Wall Growth Control 100
Introduction 100
Initial Growth State of Smooth Muscle Cells 101
Initial Growth State of Endothelial Cells 104
Interrelationships of Endothelial and Smooth Muscle Growth 108
Critical Lesion Size 111
Summary 112
References 113
Chapter 4. Angiogenesis in Vitro: Implications for Tumor Biology 116
Introduction 116
Method of Isolation of Capillary Endothelial Cells 117
Angiogenesis in Vitro 120
Time-Lapse Studies 123
Other Factors Affecting Growth of Capillary Endothelial Cells 123
Migration of Capillary Endothelial Cells 124
The Effect of Mast Cells on Migration of Capillary Endothelial Cells 126
Summary 129
References 129
Part II: Coagulation Proteases 132
Chapter 5. Interactions of Thrombin with Endothelium 134
Text 134
References 138
Chapter 6. Protein C, a Regulator of Prothrombin Activation 140
Introduction 140
Human Protein C 144
Factor V 146
Protein Ca and Factor V 150
Protein C and Platelets 151
References 155
Chapter 7. Activation of Protein C by a Complex between Thrombin and Endothelial Cell Surface Protein 158
Introduction 158
Materials and Methods 160
Results 161
Discussion 167
References 172
Part III: Platelet Adhesion and Fibrinogen Proteolysis 174
Chapter 8. The Role of Factor VIH-von Willebrand Factor in Adhesion of Platelets to the Vessel Wall 176
Introduction 176
Methods 177
Results and Discussion 179
Conclusions 187
References 188
Chapter 9. The Factor VIII Complex 190
The Two Components of the Factor VIII Complex 190
Factor VIII Procoagulant Protein: Antihemophilic Factor 192
Factor VIII-Related Protein: von Willebrand Factor 193
The Interaction of VIII:C and VIIIR in the Factor VIII Complex 195
The Factor VIII Deficiency Diseases 197
VIIIR Synthesis by Endothelial Cells 198
VIII:C Synthesis 201
Summary 201
References 202
Chapter 10. Fibrinolytic Components of Cultured Endothelial Cells 204
Introduction 204
Vascular Fibrinolysis 205
Fibrinolytic Components of Cultured EC 208
Regulation of EC-Mediated Fibrinolysis 211
Discussion 215
References 218
Chapter 11. Regulation of Fibrinolysis: Plasminogen Activator as a Thrombolytic Agent 220
Introduction 220
Regulation and Control of Fibrinolysis 221
Studies on the Development of Human Tissue Plasminogen Activator as a Thrombolytic Agent 223
References 225
Chapter 12. Proteases and Angiogenesis: Production of Plasminogen Activator and Collagenase by Endothelial Cells 228
Introduction 228
Methods 229
Results 229
Discussion 233
References 234
Part IV: Endothelial Protein Synthesis 236
Chapter 13. Synthesis and Secretion of Thrombospondin and Fibroneetin by Cultured Human Endothelial Cells 238
Introduction 238
Methods 239
Results 243
Discussion 246
References 248
Chapter 14. Synthesis and Secretion of Structural Macromolecules by Endothelial Cells in Culture 252
Introduction 252
Collagen Synthesis 253
Concluding Remarks 264
References 264
Chapter 15. Glycoprotein Synthesis and Endothelial Cell Function: Influence of Thrombin and Melittin on Release of Glycoconjugates, Activity of Converting Enzyme, and Cell Morphology 266
Introduction and Review 266
Studies with Matrix Components and Endothelial Cells 271
Methods 271
Results 274
Discussion 280
References 283
Part V: Prostaglandins 288
Chapter 16. The Role of Prostaglandins in Platelet-Vessel Wall Interactions 290
Introduction 290
Pathways of Arachidonic Acid Metabolism 291
Prostacyclin 294
Thromboxane A2 300
Prostacyclin and Thromboxane A2 Interactions in Hemostasis and Thrombosis 301
Factors Affecting Prostacyclin Formation 302
Prostacyclin in Man 308
Therapeutic Potential of Prostacyclin 308
References 313
Chapter 17. Prostaglandins, Thromboxanes, and Leukotrienes 324
Text 324
References 335
Chapter 18. Synthesis of Prostacyclin by Cultured Endothelial Cells 338
Introduction 338
Induction of PGI2 Synthesis 339
Inhibitors of PGI2 Synthesis 340
References 343
Chapter 19. Essential Fatty Acids in Platelets and Endothelial Cells 348
Introduction 348
Arachidonic Acid Metabolites as Autacoids 349
Arachidonic Acid Metabolism in Human Platelets 349
Current Concepts of Arachidonic Acid Mobilization 350
Metabolism of Arachidonic Acid and Prostaglandin Endoperoxide PGH2 in Cultured Human Endothelial Cells 351
Synthesis of Prostacyclin from Platelet-Drived PGH2 by Cultured Human Endothelial Cells 352
Studies with Acetyl Glyceryl Ether Phosphorylcholine (AGEPC), Formerly Known as Platelet Activating Factor (PAF) 355
References 357
Chapter 20. Role of Injury in Arterial Lipid Metabolism 360
Text 360
References 371
Part VI: ENDOTHELIAL CELL-CELL INTERACTIONS 374
Chapter 21. Interactions of Platelets with Endothelial Cells 376
Introduction 376
Adhesion of Platelets to Cultured Cells 379
Endothelial Cell Production of Prostacyclin 381
Secretion of ADP by Endothelial Cells 382
Binding of Platelet Proteins to Endothelial Cells 383
Conclusions and Questions 385
References 386
Chapter 22. Secretory Products of Mononuclear Phagocytes 388
Text 388
References 393
Chapter 23. Leukocyte-Endothelial Interactions 394
Text 394
References 404
Chapter 24. Complement-Mediated Intravascular Leukostasis and Endothelial-Cell Injury 406
Introduction 406
Granulocyte Aggregation and Hemodialysis Leukopenia 408
Organ Dysfunction Associated with Intravascular Granulocyte Aggregation 411
Granulocyte Aggregation and Shock Lung 413
The Reversibility of Hemodialysis Leukopenia 416
Conclusions 420
References 421
Chapter 25. T-Lymphocyte Activation by Antigen Presented by HLA-DR Compatible Endothelial Cells 424
Introduction 424
Materials and Methods 425
Results and Discussion 428
References 443
Chapter 26. A Recognition Function of Endothelial Cells: Directing Lymphocyte Traffic 446
Introduction 446
Methods 448
Results and Discussion 450
Summary 460
References 461
Chapter 27. Pulmonary Endothelial Cell Injury Induced by Antibody Fragments to Angiotensin Converting Enzyme 462
Text 462
References 467
Part VII: Vascular Pathobiology 468
Chapter 28. Responses of Endothelial Cells to Injury 470
Introduction 470
Membrane Permeability and the Effects of Injurious Stimuli: Possible Mechanisms Involved 471
Endothelial Injury in Vivo: Consequences of Endothelial Removal 475
Endothelial Injury in Vivo: Observations on Cultured Cells 478
Conclusions 488
References 489
Chapter 29. Vital and Functional Activities of Endothelial Cells 492
Introduction 492
Vital Activities 494
Functional Activities 496
Responses of Pulmonary Endothelial Cells to the Vasoactive Hormones, Bradykinin and Angiotensin II 499
Conclusions 504
References 505
Chapter 30. Endothelial Cell Function in Altered Oxygen Environments 508
Text 508
References 521
Index 524

Produkt-Details

• Erscheint lt. Verlag: 22.10.2013
• Sprache: englisch
• Themenwelt: Sachbuch/Ratgeber ► Gesundheit / Leben / Psychologie ► Krankheiten / Heilverfahren
• Themenwelt: Medizin / Pharmazie ► Medizinische Fachgebiete
• Themenwelt: Naturwissenschaften ► Biologie
• Themenwelt: Technik
• ISBN-10: 1-4832-1896-1 / 1483218961
• ISBN-13: 978-1-4832-1896-0 / 9781483218960