Protides of the Biological Fluids (eBook)

Proceedings of the Nineteenth Colloquium, Bruges, 1971

H. Peeters (Herausgeber)

eBook Download: PDF

2016 | 1. Auflage
606 Seiten
Elsevier Science (Verlag)
978-1-4831-4634-8 (ISBN)

Infos

Protides of the Biological Fluids is a compendium of papers presented at the XIX Colloquium held at Bruges in 1971. It focuses on three main topics: lipoproteins, proteins, and protein catabolism. The main section of this book contains 60 papers discussing the composition, structure, synthesis, genetics, and function of lipoproteins. Another section is devoted to dielectric relaxation, fluorescence depolarization and determination of protein structure. The final section discusses protein catabolism and the applications of immunoelectrophoresis to protein quantitation. Students and scientists looking for an extensive reference on protein chemistry will find this book invaluable.

Inhalt

Front Cover 1
Protides of the Biological Fluids 4
Copyright Page 5
Table of Contents 6
Preface 14
Acknowledgements 16
ACADEMIC LECTURE 18
Chapter 1. Immunochemical Studies on Human Gastro-intestinal Tumors 20
REFERENCES 22
SECTION A: Lipoproteins 24
Part A: Introduction 26
Chapter 2. Conceptual Development of the Classification Systems of Plasma Lipoproteins 26
OPERATIONAL CLASSIFICATION SYSTEMS OF PLASMA LIPOPROTEINS 26
CHEMICAL CLASSIFICATION SYSTEM OF PLASMA LIPOPROTEINS 29
ACKNOWLEDGEMENTS 35
REFERENCES 35
Part A1: Composition and Structure 38
Chapter 3. Isolation of Lipoproteins from Human Serum by Precipitation with Polyanions and Divalent Cations 38
FRACTIONATION OF SERUM LIPOPROTEIN BY POLYANIONS 38
ABNORMAL LIPOPROTEIN OF JAUNDICED SERUM—NATURE OF THE INTERACTION LIPOPROTEIN-POLYANION-CATION 41
ISOLATION OF LIPOPROTEINS FROM HUMAN SERUM'3' 42
ISOLATION OF LDL PLUS VLDL AND HDL BY PRECIPITATION WITH DEXTRAN SULFATE AND MnCl2 (3) 43
ANALYTICAL STUDIES 44
SUMMARY 44
REFERENCES 44
Chapter 4. A Comparison between HDL Lipoproteins and Apoprotein- Phosphatidylcholine Cenapses 46
INTRODUCTION 46
MATERIALS AND METHODS 46
RESULTS 47
DISCUSSION 49
REFERENCES 50
Chapter 5. Comparative Studies on Different Lipid-staining Procedures, Especially for Cellulose Acetate Electrophoresis 52
INTRODUCTION 52
THE CONVENTIONAL FAT-SOLUBLE DYE-STAINING METHODS 52
OZONE-SCHIFF STAINING METHOD 53
CONCLUSION 56
REFERENCES 57
Chapter 6. Characteristics of Six Fractions Obtained from Human High Density Lipoprotein 58
ACKNOWLEDGEMENTS 62
REFERENCES 62
Chapter 7. Electrofocusing of Normal Apolipoproteins and the Amino Acids of Their Subfractions 64
INTRODUCTION 64
MATERIALS AND METHODS 64
RESULTS 65
DISCUSSION 69
ACKNOWLEDGEMENT 69
REFERENCES 70
Chapter 8. Studies on the "Native" and the in Vitro-obtained Apoprotein of Duck Serum HDL 72
REFERENCES 75
Chapter 9. Identification of Lipoprotein Families in High Density Lipoproteins of Normal Human Plasma 76
SUMMARY 79
REFERENCES 80
Chapter 10. The Use of Streptolysin O as a Macromolecular Marker of Free Cholesterol in the ß-Lipoprotein Molecule 82
RESULTS 84
ACKNOWLEDGEMENT 85
REFERENCES 86
Chapter 11. Salting-in Gel Chromatographic Separation of Serum Low Density Lipoproteins on Sephadex Columns 88
MATERIALS AND METHODS 88
RESULTS AND DISCUSSION 89
REFERENCES 91
Chapter 12. Lipid Composition of Lipoprotein Fractions 92
REFERENCES 96
Chapter 13. Studies on the Proteins of Cat Lymph Chylomicrons 98
REFERENCES 101
Chapter 14. Role of Serum Lipoproteins in Hemolysis Induced by Vipera aspis Venom Phospholipases 102
MATERIALS AND METHODS 102
RESULTS 102
SUMMARY 107
REFERENCES 107
Chapter 15. Agarose Gel, Cellulose Acetate, and Polyacrylamide Gel Lipoprotein Electrophoresis—A Comparison of Methods 108
METHODS 108
RESULTS 109
SUMMARY AND CONCLUSIONS 111
ACKNOWLEDGEMENTS 111
REFERENCES 111
Chapter 16. Staining of Lipoproteins in Cellulose Acetate Membrane 114
TECHNICAL DETAILS 116
REFERENCE 116
Chapter 17. Oxygenated Sterol Esters of Human Atherosclerotic Plaques 118
INTRODUCTION 118
METHODS 118
RESULTS 118
DISCUSSION 123
ACKNOWLEDGEMENTS 123
REFERENCES 123
Part A2: Synthesis and Degradation 126
Chapter 18. Comparative Studies on the in vitro Effects of Delipidation with Organic Solvents, Enzymatic Lipolysis, Action of 'Anionic, Non-ionic and Cationic Detergents and the in vivo Effect of Lipoproteinlipase on Duck Serum a-Lipoprotein 126
REFERENCES 133
Chapter 19. Study of the Human Serum ß-Lipoprotein Antigenicity according to Its Degree of Delipidation 134
DELIPIDATION METHODS 134
RESULTS 135
DISCUSSION 136
CONCLUSION 137
REFERENCES 137
Chapter 20. Influence of Cholesterol on the Concentration, Composition and Synthesis of Chicken Serum Lipoproteins 138
SUMMARY 142
REFERENCES 142
Chapter 21. Isovaleric Acid in Acoustic Tissues of Porpoises: Triacylglycerols Resistant to Porcine Pancreatic Lipase 144
ACKNOWLEDGEMENT 146
REFERENCES 146
Chapter 22. Effect of Clofibrate on Lipoprotein Metabolism 148
ACKNOWLEDGEMENTS 151
REFERENCES 151
Chapter 23. Biosynthesis of Plasma Glycolipoproteins 152
GLYCOSYLATION OF PLASMA LIPOPROTEINS 152
EFFECT OF CDP-CHOLINE ON N-ACETYLGLUCOSAMINYL TRANSFERASE IN VARIOUS SUB-CELLULAR FRACTIONS 153
EFFECT OF DIFFERENT LIPID FACTORS ON N-ACETYLGLUCOSAMINYL TRANSFERASE ENZYME 153
MECHANISM OF CDP-CHOLINE ACTION 154
EFFECT OF CDP-CHOLINE ON GLYCOSYLATION OF THE j8-LIPOPROTEINS 155
ACKNOWLEDGEMENTS 155
REFERENCES 155
Chapter 24. Human Intestinal Lipoprotein Particle Synthesis and Transport 158
SUMMARY 162
REFERENCES 164
Chapter 25. The Metabolism of the Plasma Cholesterol in Man 166
WHOLE-BODY EXCHANGEABLE CHOLESTEROL 166
PLASMA CHOLESTERYL ESTERS 166
TURNOVER OF THE EXCHANGEABLE MASS OF CHOLESTEROL 168
CHANGES IN TURNOVER OF THE PLASMA CHOLESTEROL 170
REFERENCES 170
Chapter 26. Metabolism of Very Low Density Lipoproteins in Diabetes Mellitus 172
REFERENCES 173
Part A3: Genetics 174
Chapter 27. The Ag-System—Present Concepts and Immunogenetic Models 174
REFERENCES 177
Chapter 28. Serology and Genetics of the Ag-System 178
REMARKS ON Ag-TYPING PROCEDURES 178
GENETICS OF THE Ag-SYSTEM 179
REMARKS ON Ag-ANTIBODIES 182
REFERENCES 183
Chapter 29. Genetic Polymorphisms of Lipoproteins 186
CONCERNING THE IMMUNOLOGY OF THE Lp SYSTEM 187
INTERPRETATIONAL PROBLEMS IN THE Lp SYSTEM 187
SOME DIFFICULTIES AND PARADOXES ENCOUNTERED IN WORK WITH THE Lp AND Ag SYSTEMS 189
SERUM LIPOPROTEINS AND CELL MEMBRANES 189
REFERENCES 194
Chapter 30. Immunological Relationship and Chemical Similarities between HDL Lipoproteins from Various Mammalian and Avian Species 196
REFERENCES 198
Chapter 31. On the Significance of the El* (BA) Variants 200
METHODS 200
RESULTS 202
DISCUSSION 206
REFERENCES 206
Chapter 32. Subunit Structure of the Lp{d) Lipoprotein 208
REFERENCES 212
Chapter 33. The Isolation and Partial Purification of Low Density Lipoproteins Carrying the Ag(x) and Ag(y) Antigenic Determinants 214
MATERIALS AND METHODS 214
RESULTS 215
ACKNOWLEDGEMENTS 217
REFERENCES 217
Chapter 34. A Serum Type of Isoprecipitin System of HD Lipoproteins 218
MATERIALS AND METHODS 218
RESULTS AND DISCUSSION 218
ACKNOWLEDGEMENT 220
REFERENCES 220
Chapter 35. Plasma Lipoprotein Distribution in Normal Children and Adults of Different Ages 222
INTRODUCTION 222
MATERIALS AND METHODS 222
RESULTS 222
DISCUSSION 225
REFERENCES 225
Part A4: Function 226
Chapter 36. The Arterial Foam Cell—in vitro and in vivo Studies 226
REFERENCES 231
Chapter 37. A New Method of Preparation for the Apoprotein Moiety from Folch-Pi Proteolipid 234
APOPROTEIN PREPARATION 234
COMPARATIVE STUDY OF THE APOPROTEINS 235
DISCUSSION 236
REFERENCES 237
Chapter 38. Evidence for a Covalent Bond between Lipid and a Membrane Protein {Rigid Layer of the Cell Wall ofE. coli) 238
REFERENCES 241
Chapter 39. Distribution of Lipoproteins in Arteries and Tissue as Determined by Immunohistological and Radioactive Tracer Methods 242
ARTERIAL LESIONS (Atherosclerosis) 242
XANTHOMATA 242
CORNEAL ARCUS FORMATION 243
DISCUSSION 243
REFERENCES 243
Chapter 40. Mechanism of Uptake of Plasma Lipoprotein Triglyceride by Avian Ovarian Follicles 244
IN VITRO SYSTEM TO STUDY LIPOPROTEIN LIPID UPTAKE BY AVIAN OOCYTES 244
IN VITRO UPTAKE OF VLDL TRIGLYCERIDE 245
EFFECT OF PROTAMINE-SULFATE AND HEPARIN ON VLDL TRIGLYCERIDE UPTAKE 246
UPTAKE OF DOUBLE-LABELED VLDL TRIGLYCERIDE 248
REFERENCES 248
Chapter 41. Uptake of Plasma Lipoproteins by the Mammary Gland ofLactating Cows 250
REFERENCES 253
Chapter 42. Cholesterol Movements between Rat Plasmatic Lipoproteins and Red Cells in vitro 254
MATERIALS AND METHODS 254
RESULTS 254
DISCUSSION 256
REFERENCES 257
Chapter 43. The Formation of Lipoproteins in Hen Liver and Egg Yolk 260
REFERENCES 264
Part A5: Pathology 266
Chapter 44. Isolation and Properties of Abnormal Serum Lipoproteins in Cholestasis 266
FRACTIONATION OF LOW DENSITY LIPOPROTEINS 266
IMMUNOLOGICAL PROPERTIES OF ABNORMAL LIPOPROTEINS 266
ABNORMAL LIPOPROTEIN PHOSPHOLIPIDS 269
REFERENCES 270
Chapter 45. Clinical Lipid Evaluations 272
SUMMARY AND CONCLUSIONS 277
ACKNOWLEDGEMENTS 277
REFERENCES 277
Chapter 46. Diagnosis of Familial Hypercholesterolaemia in Infancy 278
PATIENTS AND METHODS 278
RESULTS 278
CONCLUSION 281
ACKNOWLEDGEMENTS 281
REFERENCES 281
Chapter 47. Dyslipidaemias in the Neonatal Period 282
INTRODUCTION 282
MATERIALS AND METHODS 282
RESULTS 283
DISCUSSION OF THE CORD BLOOD RESULTS 287
SUMMARY 288
ACKNOWLEDGEMENTS 288
REFERENCES 288
Chapter 48. Human Myeloma Proteins which Bind ß-Lipoproteins 290
INTRODUCTION 290
SCREENING FOR BINDING ACTIVITY AGAINST LIPOPROTEINS 290
CORRELATION BETWEEN BINDING ACTIVITY AND SERUM LIPID CONCENTRATION 291
CHARACTERIZATION AND STUDIES ON THE SPECIFICITY OF AN IgA MYELOMA PROTEIN 292
RELATION BETWEEN MYELOMA PROTEIN AND LIPID CONCENTRATION IN SERUM (L.W.) 292
DISCUSSION 294
ACKNOWLEDGEMENTS 295
REFERENCES 295
Chapter 49. Primary Hyperlipoproteinaemias in Myocardial Infarction 296
REFERENCES 299
Chapter 50. Type III Hyperlipoproteinaemia with Xanthomatosis 300
METHODS 300
RESULTS 301
REFERENCES 304
Chapter 51. A Simple Method for the Detection and Identification of Various Types of Hyperlipoproteinemia 306
RESULTS 307
SUMMARY 309
REFERENCES 309
Chapter 52. Problem Cases in Phenotyping 310
THE BASIS OF PHENOTYPING 310
RANGES OF THE DISCRIMINATING PARAMETERS 310
SHIFTS IN PHENOTYPE 312
BORDERLINE PATHOLOGY 312
Chapter 53. Phenotyping of Hyperlipidaemias by Electrochromatography 316
INTRODUCTION 316
MATERIAL AND METHODS 316
RESULTS 317
DISCUSSION 321
ACKNOWLEDGEMENT 322
REFERENCES 322
Chapter 54. Lipid Electrophoresis on Cellulose Acetate Membranes and on Agarose 324
SUMMARY 327
REFERENCES 327
Chapter 55. Quantitative Lipoprotein andLipid Determinations in Patients with Various Malignancies 328
INTRODUCTION 328
MATERIAL AND METHODS 328
RESULTS 328
DISCUSSION 331
REFERENCES 331
Chapter 56. A General Review of Clinical and Laboratory Features of Familial Hypercholesterolemia (Type II Hyperbetalipoproteinemia) 332
THE HOMOZYGOTES 332
THE HETEROZYGOTES 334
GENETICS 334
DISCUSSION 335
ACKNOWLEDGEMENT 335
REFERENCES 335
Part A6: Dietary Measures and Drug Therapy 336
Chapter 57. The Validity of Animal Models in the Study of Atherogenesis 336
INTRODUCTION 336
THE SELECTION OF A SUITABLE ANIMAL MODEL 336
A BRIEF REVIEW OF EXPERIMENTALLY INDUCED ATHEROSCLEROSIS 337
Chapter 58. Dietary and Drug Management of Familial Hypercholesterolemia 340
RESULTS 340
DISCUSSION 343
ACKNOWLEDGEMENT 343
REFERENCES 343
Chapter 59. Oxidative Metabolism in Normal and Hyperlipemic Patients 344
MODEL 344
CALCULATIONS ASSOCIATED WITH THE MODEL 345
REFERENCES 346
Chapter 60. Management of Abetalipoproteinaemia in Childhood 348
CONTROL OF MALABSORPTION AND MAINTENANCE OF GROWTH 348
VITAMIN SUPPLEMENTATION AND POSSIBLE PREVENTION OF NEUROLOGICAL AND RETINAL LESIONS 348
ACKNOWLEDGEMENTS 352
REFERENCES 352
Chapter 61. Dietary and Hormonal Modifications of Rat Serum Lipoproteins 354
REFERENCES 356
Chapter 62. The Lipoproteins in Hyperlipidaemic Primates as a Model for Human Atherosclerosis 358
LIPOPROTEINS IN BABOONS GIVEN AN ATHEROGENIC DIET 358
TYPE II HYPERLIPOPROTEINAEMIA 358
COMPARISON BETWEEN MAN, BABOON AND CHIMPANZEE 359
CONCLUSIONS 360
REFERENCES 360
SECTION B: Hydrodynamic Properties of Proteins 362
Part B1: Dielectrics 364
Chapter 63. The Role of Dielectric Studies in the Investigation of Protein Molecules 364
ABSTRACT 364
INTRODUCTION 364
DISCUSSION 365
GENERAL CONCLUSIONS 369
REFERENCES 369
Chapter 64. The Dipole Moment of Urea and Thiourea in Water and Ethane Diol 372
RESULTS AND DISCUSSION 372
REFERENCES 374
Chapter 65. Measurement of the Hydration of Biological Molecules from Dielectric Measurements at Centimetre Wavelengths 376
INTRODUCTION 376
METHODS AND MATERIALS 376
RESULTS 377
DISCUSSION 378
CONCLUSIONS 383
ACKNOWLEDGEMENT 383
REFERENCES 383
Chapter 66. Recent Progress in the Dielectric Research on Polynucleotides 384
DIELECTRIC STUDIES OF DNA IN SHEAR GRADIENT 384
DIELECTRIC RELAXATION OF SYNTHETIC POLYNUCLEOTIDES 386
ACKNOWLEDGEMENT 387
REFERENCES 387
Chapter 67. Permittivity Measurements on Biological Solutions at Very High Frequency 388
INTRODUCTION 388
THEORETICAL 388
EXPERIMENTAL 390
RESULTS 392
CONCLUSION 393
REFERENCES 393
Chapter 68. Deduction of Size and Hydration of Protein Molecules from Permittivity Measurements 394
INTRODUCTION 394
THEORETICAL 394
MATERIALS AND METHODS 395
RESULTS 395
CONCLUSION 398
REFERENCES 399
Part B2: Fluorescence 400
Chapter 69. The Use of Fluorescence Techniques for Studying the Structure and Structural Transitions of Polypeptides in Solution 400
TECHNIQUE 400
UREA EFFECTS 401
MOLECULAR TRANSITIONS 402
SUMMARY 409
REFERENCES 409
Chapter 70. The Decay of the Fluorescence Anisotropy of Biological Macromolecules 410
INTRODUCTION 410
THEORETICAL ASPECT 410
EXPERIMENTAL DETAILS 412
APPLICATION OF THE METHOD 412
REFERENCES 414
Chapter 71. The Influence of the Microenvironments of the Tryptophan Groups of Proteins upon Their Fluorescence Properties 416
REFERENCES 418
Chapter 72. Determination of Fluorescence Lifetimes 420
METHOD 420
RESULTS 421
REFERENCES 424
Chapter 73. Hydrodynamic Studies of Some Lysozymes and a-Lactalbumins 426
INTRODUCTION 426
EXPERIMENTAL PROCEDURE 426
RESULTS AND DISCUSSION 427
SUMMARY 428
ACKNOWLEDGEMENTS 428
REFERENCES 428
SECTION C: Protein Catabolism 430
Chapter 74. Catabolism and Distribution of IgG in Different Diseases 432
MATERIAL 432
METHODS 432
RESULTS 433
DISCUSSION 438
ACKNOWLEDGEMENT 440
REFERENCES 440
Chapter 75. Disorders of Immunoglobulin Catabolism 442
INTRODUCTION 442
ENDOGENOUS HYPERCATABOLISM AFFECTING A SINGLE PROTEIN 442
ENDOGENOUS HYPERCATABOLISM AFFECTING SEVERAL SERUM PROTEINS 443
SUMMARY 444
REFERENCES 444
Chapter 76. Thermodynamics of the Denaturation of Human Serum Albumin in Relation to Intra-vital Breakdown 446
INTRODUCTION 446
MATERIALS AND METHODS 448
RESULTS AND DISCUSSION 448
CONCLUSION 452
REFERENCES 453
Chapter 77. Plasma Protein Catabolism in the Capillary Wall? 454
INTRODUCTION 454
METHODS 455
RESULTS 455
DISCUSSION 455
SUMMARY 458
REFERENCES 458
Chapter 78. IgM Degradation in Ménétrier's Disease (Giant Hypertrophic Gastritis) 460
METHODS 460
CASE MATERIAL 461
RESULTS AND DISCUSSION 461
REFERENCES 464
Chapter 79. IgA Metabolism in Control Subjects, in Patients with Elevated IgA Levels and in Patients with IgA Deficiency 466
INTRODUCTION 466
PATIENTS 466
METHODS 468
RESULTS 468
DISCUSSION 469
ACKNOWLEDGEMENT 469
REFERENCES 470
Chapter 80. Possible Enhancement oflgM Catabolism by D-Penicillamine 472
CASE SUMMARY 474
DISCUSSION 474
SUMMARY 476
REFERENCES 476
Chapter 81. The Catabolism of Human .G-Globulin and Its Fragments in Man and Mouse 478
METHODS 478
RESULTS 479
DISCUSSION 480
ACKNOWLEDGEMENTS 482
REFERENCES 482
Chapter 82. A Consideration of Short-term Measurement of Plasma Protein Catabolic Rates 484
CATABOLIC RATE MEASUREMENTS IN THIS STUDY 484
REFERENCES 488
Chapter 83. Rate of Synthesis of Albumin during the Early Postoperative Period 490
INTRODUCTION 490
PREOPERATIVE MODEL 490
POSTOPERATIVE MODEL 491
CONCLUSION 493
REFERENCES 493
Chapter 84. The Isolated Perfused Liver in the Study of Albumin Synthesis 494
DISCUSSION 497
ACKNOWLEDGEMENTS 498
REFERENCES 498
Chapter 85. Albumin Metabolism in Patients with Chronic Renal Failure on Low Protein Balanced Diet 500
INTRODUCTION 500
MATERIALS AND METHODS 500
RESULTS 501
CONCLUSIVE REMARKS 503
REFERENCES 504
Chapter 86. Regulation of Transferrin Synthesis 506
INTRODUCTION 506
METHODS 506
EXPERIMENTS AND RESULTS 507
DISCUSSION 509
ACKNOWLEDGEMENTS 510
REFERENCES 510
Chapter 87. Catabolism of Nonmitochondrial Glutamic Oxalacetic Transaminase from Pig Heart in Piglets 512
REFERENCES 516
Chapter 88. The Turnover of Lysozyme 518
MATERIALS AND METHODS 518
RESULTS AND DISCUSSION 518
REFERENCES 521
SECTION D: New Methods 522
Chapter 89. Studies of Protein Interactions by Microcalorimetry 524
MICROCALORIMETERS 525
THERMOCHEMICAL STUDIES OF PROTEIN BINDING REACTION 527
REFERENCES 529
Chapter 90. Isotachophoresis in Capillary Tubes 530
INTRODUCTION 530
SEPARATION PRINCIPLES 530
DETECTION METHODS 532
EXPERIMENTAL 533
SEPARATIONS 533
DISCUSSION 536
REFERENCES 536
Chapter 91. The Isolation of Human Transferrin by Isotachophoresis 538
INTRODUCTION 538
FRACTIONATION OF SERUM 538
RESULTS 540
CONCLUSIONS 540
ACKNOWLEDGEMENTS 541
REFERENCES 541
Chapter 92. Oil-cooled Apparatus for Quantitative Immunoelectrophoresis 542
EXPERIMENTAL 542
DISCUSSION 543
ACKNOWLEDGEMENTS 544
REFERENCES 544
Chapter 93. Quantitative Immunoelectrophoresis Applied to the Analysis of Elution Profiles in Column Chromatography 546
MATERIALS AND METHODS 546
COMBINATION OF LINE- AND CROSSED-IMMUNOELECTROPHORESIS 546
COMBINATION OF LINE- AND ROCKET IMMUNOELECTROPHORESIS 546
RESULTS 547
DISCUSSION 549
ACKNOWLEDGEMENTS 549
REFERENCES 549
Chapter 94. A Modified Radioimmunoassay for Quantitative Determination of IgG Subclasses in Man 550
MATERIALS AND METHODS 550
RESULTS AND DISCUSSION 552
ACKNOWLEDGEMENTS 555
REFERENCES 557
Chapter 95. Immunoselection Technique and Its Applications 558
SUMMARY 561
REFERENCES 562
Chapter 96. Storage of Human Serum for Immunochemical Determination of the Serum Proteins 564
INTRODUCTION 564
METHODS 564
RESULTS AND COMMENTS 564
CONCLUSION 569
REFERENCES 569
Chapter 97. Enzyme-linked Immunosorbent Assay, ELISA 570
QUANTITATIVE DETERMINATION OF RABBIT IgG 570
DETERMINATION OF ANTIBODIES IN SERUM 571
ACKNOWLEDGEMENTS 573
REFERENCES 573
Chapter 98. Determination of Insulin Binding to ß-Lipoprotein in Sera from Normals and Diabetics Applying Immunoelectrophoresis 574
CONCLUSIONS 577
REFERENCES 577
Chapter 99. Candida Precipitins Characterized by a Modified Antigen-Antibody Crossed Electrophoresis 578
INTRODUCTION 578
MATERIALS AND METHODS 578
RESULTS AND DISCUSSION 579
SUMMARY 581
ACKNOWLEDGEMENT 581
REFERENCES 581
Chapter 100. An Automated System of Two-dimensional Immunoelectrophoresis 582
TECHNIQUE 582
ACKNOWLEDGEMENT 585
REFERENCES 585
Chapter 101. Micro-polyacrylamide Flat Gel Electrophoresis: Advantages and Applications 586
INTRODUCTION 586
METHODS 586
RESULTS 588
DISCUSSION 589
ACKNOWLEDGEMENTS 590
REFERENCES 590
Chapter 102. Comparison of Slab and Cylinder Gel Focusing 592
ACKNOWLEDGEMENTS 595
REFERENCES 595
Chapter 103. Studies of Aggregates of lgG in Human Sera and Commercial .-Globulin Preparations with a New Immuno-gel Filtration Method 596
MATERIAL AND METHODS 596
RESULTS 596
DISCUSSION 601
SUMMARY 601
REFERENCES 601
Author Index 602
Subject Index 604

Produkt-Details

• Erscheint lt. Verlag: 20.4.2016
• Sprache: englisch
• Themenwelt: Studium ► 1. Studienabschnitt (Vorklinik) ► Physiologie
• Themenwelt: Naturwissenschaften ► Biologie ► Humanbiologie
• Themenwelt: Technik
• ISBN-10: 1-4831-4634-0 / 1483146340
• ISBN-13: 978-1-4831-4634-8 / 9781483146348