Immuno-Gold Electron Microscopy in Virus Diagnosis and Research

Alexander D. Hyatt, Bryan Eaton (Autoren)

Buch | Hardcover

464 Seiten

1992
Crc Press Inc (Verlag)
9780849367595 (ISBN)

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This book presents a wide variety of immuno-gold techniques for use in virus diagnosis and research. Protocols are presented for state-of-the-art techniques, including in situ hybridization, freeze substitution, and the utilization of ultra-small probes and replicas for use by virologists and electron microscopists identifying and studying viruses, their components, and replication in cells. The procedures are described by eminent scientists and are pertinent to both experienced researchers and newcomers to this field who are interested in the localization of low antigenic mass structures.

Alexander D. Hyatt, Bryan Eaton

Immuno-Gold Electron Microscopy for Virus Diagnosis: Immune Complexing (E.L. Palmer and M.L. Martin). Solid-Phase Immune Electron Microscopy of Virus Preparations (R.G. Milne). Gold Labeling in Negative Stain Immune Electron Microscopy (P.J.C. Dopping-Hepenstal and J.E. Beesley). The Grid-Cell-Culture Technique (A.D. Hyatt and B.T. Eaton). Use of Microspheres for Immune Scanning Electron Microscopy (J.P. Andersson, U.G. Andersson, and A. Sonnerborg). Immuno-Gold Electron Microscopy in Virus Research: The Colloidal Gold Probe, An Overview of Its Application in Viral Cytochemistry (S. Garzon and M. Bendayan). Detection of Viral and Cellular Structures by Post-Embedding Immunocytochemistry (G.N. Williams and P. Faulkner). Multiple Labeling of Thin Sections (M. Nielsen and L. Bastholm). Immuno-Gold Replica Technique for Study of Virus-Infected Cells (K. Mannweiler, H. Hohenberg, G. Rutter, and W. Bohn). Detection and Analysis of Viral Proteins by Immuno-Gold Labeling (K.G. Murti). Cryo-Sectioning (H.K. Hagler). Improvements of Immuno Dection Using Ultra-Small Gold Probes and Cryosections (J.L.M. Leunissen and P. van de Plas). Freeze Substitution in Virus Research: A Preview (H. Schwartz, H. Hohenberg, and B.M. Humbel). Ultrastructural In Situ Hybridization Using Immuno-Gold (G.L. Bassell and R.H. Singer). Use of Colloidal Gold and Silver Enhancement in Scanning Electron Microscopy (A.D. Hyatt and S.M. Brookes).

Erscheint lt. Verlag	10.11.1992
Zusatzinfo	16 Tables, black and white
Verlagsort	Bosa Roca
Sprache	englisch
Maße	156 x 234 mm
Gewicht	1020 g
Themenwelt	Studium ► 2. Studienabschnitt (Klinik) ► Pathologie
	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
ISBN-13	9780849367595 / 9780849367595
Zustand	Neuware