In Vitro Methods in Cell-Mediated Immunity (eBook)

Barry R. Bloom, Philip R. Glade (Herausgeber)

eBook Download: EPUB

2014 | 1. Auflage
602 Seiten
Elsevier Science (Verlag)
978-1-4832-7759-2 (ISBN)

Infos

In Vitro Methods in Cell-Mediated Immunity focuses on methods for approaching cell-mediated immune responses in vitro. This book provides, in simplified in vitro systems, a basis for understanding the mechanism of the in vivo response and discusses useful and reliable in vitro tests for cell-mediated immune responses in humans, where in vivo testing is often not possible. The topics discussed include factors and activities produced in vitro by lymphocytes; biological implications of in vitro phenomena; and requirements and prospects for improved methodology. The leucocyte migration technique for in vitro detection of cellular hypersensitivity in man; proliferation of human blood lymphocytes stimulated by antigen in vitro; and virus plaque assay for antigen-sensitive are also elaborated in this text. This publication is a good reference for microbiologist and immunologists, including medical students researching on in vitro models for cell-mediated immune reactions.

Inhalt

Front Cover 1
In Vitro Methods in Cell-Mediated Immunity 4
Copyright Page 5
Table of Contents 6
PARTICIPANTS 12
CONTRIBUTORS 16
PREFACE 22
INTRODUCTORY NOTE 24
SECTION A: SESSIONS 26
Session I. Methods for Study of Mediators 28
Session II. Factors and Activities Produced in Vitro By Lymphocytes 120
Session 
176 
Session 
230 
SECTION B: METHODS 258
INDEX OF ASSAYS AND SPECIES 259
Chapter 1. The Assay of Inhibition of Macrophage Migration and the Production of Migration Inhibitory Factor (MIF) and Skin Reactive Factor (SRF) in the Guinea Pig 260
I. Materials and Equipment 260
II. Method 261
III. Preparation of Migration Inhibitory Factor from Peritoneal Lymphocytes 266
IV. Preparation of Skin Reactive Factor 269
References 271
Chapter 
274 
I. Materials & Equipment
II. Methods 276
Chapter 
284 
I. Materials & Equipment
II. Methods 285
References 286
Chapter 
288 
I. EQUIPMENT 289
II. CULTURE MEDIA AND SUPPLEMENTS 290
III. PREPARATION OF SPLEEN FRAGMENTS 290
IV. CULTIVATION OF SPLEEN FRAGMENTS 291
V. EVALUATION OF THE RESULTS 291
VI. CALCULATION OF THE CYTOTOXIC INDEX (C.I.) 292
VII. METHOD UTILIZING ARTIFICIAL FRAGMENTS 292
COMMENTS 293
NOTES 295
References 296
FOOTNOTES 297
Chapter 
298 
I. Materials & Equipment
II. Methodology 299
References 304
Chapter 
306 
I. Materials and Equipment 306
II. Preparation of Lymphocytes 307
Critical Comments 311
References 312
Chapter 
314 
I. Materials and Equipment 314
II. Methods 315
Critical Comments 317
References 319
Chapter 
322 
I. Materials 322
II. Method 323
Critical Comments 324
References 325
Chapter 
326 
I. Materials & Equipment
II. Methods 327
Critical Comments 330
References 330
Chapter 10. The Use of Cultured Lymphoid Cells as Target Cells for the Detection of Migration Inhibitory Factor 332
I. Materials and Equipment 332
II. Methods 334
Critical Comments 336
References 337
Chapter 11. An in Vitro Test for Delayed Hypersensitivity: Macrophage Spreading Inhibition (M.S.I.) 338
I. Materials and Equipment 338
II. Methods 340
References 342
Chapter 
344 
I. Materials 344
II. Methods 345
III. Modified Method for Observation of Macrophage Aggregation 348
Critical Comments 349
References 350
Chapter 
352 
I. Materials & Equipment
II. Preparation of Method 353
Critical Comments 355
References 356
Chapter 
358 
I. Materials and Equipment 358
II. Preparation of Lymphotoxin 360
III. Assay System 363
Critical Comments 366
Chapter 15. Determination of Lymphocyte-Mediated Cytotoxicity by Release of Radioactivity from 14C-Thymidine Labelled Cells 368
I. Materials and Equipment 369
II. Method 371
References 376
Chapter 
378 
I. Materials & Equipment
II. Preparation of Assay Method 379
III. Sample Calcuation 381
Critical Comments 382
References 383
Chapter 17. 51Cr-Release from Chicken Erythrocytes: An Assay System for Measuring the Cytotoxic Activity of 'Nonspecifically' Activated Lymphocytes in Vitro 386
I. Materials and Equipment 386
II. Method 388
References 393
Chapter 
394 
I. Materials and Methods 394
References 397
Chapter 
400 
I. Materials & Equipment
II. Methodology 401
References 404
Chapter 20. Production and Assay of a Lymphocyte Derived 'Proliferation Inhibitory Factor' (PIF) 406
I, Materials & Equipment
II. Preparation of Methods 412
III. Assay Systems 416
Critical Comments 421
References 424
Chapter 
426 
I. Materials 6e Equipment 426
II. Fibroblast Maintenance 427
III. Preparation of sensitized lymphocytes and confrontation with target fibroblasts 429
IV. Preparation of cytotoxic factor and addition to target cells 430
V. Evaluation of fibroblast survival with the Counter Counter 430
Critical Comments 431
References 432
Chapter 
434 
I. Method for colony inhibition test 434
III. Test for humoral cytotoxic antibody 435
III. Test for lymphocyte mediated cellular immunity 436
IV. Test for serum blocking factors abrogatingly mphocyte mediated cellular immunity 437
V. Cytotoxicity tests using Falcon #3040 microtest plates 438
Chapter 
440 
I. Materials and Equipment 440
II. Methods 442
Critical Comments 446
References 447
Chapter 
448 
I. Materials and Equipment 448
II. Methods 450
Critical Comments 456
References 457
Chapter 
458 
I. Materials & Equipment
II. Preparation of Method 459
Critical Comments 462
References 463
Chapter 
466 
I. Materials and Methods 466
References 467
Chapter 
468 
I. Materials & Equipment
II. Methodology 471
Critical Comments 475
References 478
Chapter 
480 
I. Materials and Equipment 480
II. Preparation of Cell Suspension 481
Ill .The Assay System 482
IV. Preparation and Labelling of the Cultures 482
V. Termination of Labelling and Measurement of Isotope Incorporation 483
Critical Comments 484
References 485
Chapter 
488 
I. Materials 6e Equipment 488
II. Methods 490
References 497
Chapter 
500 
I. Materials & Equipment
II. Preparation and Assay Methods 501
Critical Comments 504
Chapter 
506 
I. Materials & Equipment
II. Preparation of Indicator Cells 507
Ill .Assay System 508
Critical Comments 509
References 511
Chapter 
514 
I. Materials and Equipment 514
II. Preparation of Human Fibroblast Monolayers 516
III. Preparation of Standard Interferon and Assay Plate Monolayers 517
IV. Human Interfer on Assay Systems Employing Fibro blast Monolayers and Bovine Vesicular Stomatitis Virus 518
Critical Comments 519
References 522
Chapter 
526 
I. Materials & Equipment
II. Methods 529
Critical Comments 536
References 537
Chapter 
540 
I. Materials and Equipment 540
II. Preparation of Method 544
Critical Comments 552
References 555
Chapter 
556 
Methods 557
References 570
Chapter 
572 
I. Materials and Equipment 572
II. Methodology 573
References 576
Chapter 
578 
I. Materials and Equipment 578
I. Miscellaneous equipment 580
II. Preparation of Lymphocytes from Sensitized Human Donors 580
III. Preparation of Cells from Tuberculin Sensitive Guinea Pi£S 580
IV. Virus Plaque Assay 581
References 584
Chapter 
586 
I. Materials 6e Equipment 587
II. Preparation and Method 588
Critical Comments 593
References 595
APPENDIX TABLES 596
ABBREVIATIONS 600
INDEX OF PARTICIPANTS 602

Textauszug

CONTRIBUTORS

William H. Adler,     Tumor Biology Unit, Department of Pathology, University of Florida, College of Medicine, Gainesville, Florida 32601

H. Bach and Fritz,     Departments of Medical Genetics and Medicine, University of Wisconsin, Madison, Wisconsin

A.C. Bausher and Judith,     Tumor Biology Unit, Department of Pathology, University of Florida, College of Medicine, Gainesville, Florida 32601

Boyce Bennett,     Department of Pathology, Albert Einstein College of Medicine, Bronx, New York 10461

Barry R. Bloom,     Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461

Susan Westerman Broder,     Westerman Department of Pediatrics, Division of Infectious Diseases, Mt. Sinai School of Medicine, New York, New York 10029

K. Theodore Brunner,     Department of Immunology, Swiss Institute for Experimental Cancer Research, Lausanne, Switzerland

Jean-Charles Cerottini,     Department of Immunology, Swiss Institute for Experimental Cancer Research, Lausanne, Switzerland

Lawrence Chessin,     Department of Medicine, University of Rochester Medical School, Rochester, New York

Sidney R. Cooperband,     Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118

John R. David,     Robert B. Brigham Hospital, 125 Parker Hill Road, Boston, Massachusetts 02115

Roberta David,     Robert B. Brigham Hospital, 125 Parker Hill Road, Boston, Massachusetts 02115

Dragan Dekaris,     Department of Experimental Pathology, Institut Pasteur, 92 Garches, France

Sheldon Dray,     Departments of Pediatrics and Microbiology, University of Illinois Medical Center, Chicago, Illinois 60612

Dudley C. Dumonde,     Division of Immunology, Kennedy Institute of Rheumatology, Hammersmith W6, London, England

Rudolf E. Falk,     Department of Surgery, Toronto University Medical School, Toronto 181, Canada

Robert M. Fauve,     Department of Experimental Pathology, Institut Pasteur, 92 Garches, France

Philip R. Glade,     Department of Pediatrics, Division of Infectious Diseases, Mt. Sinai School of Medicine, New York, New York 10029

Samuel P. Gotoff,     Departments of Pediatrics and Microbiology, University of Illinois Medical Center, Chicago, Illinois 60612

Gale A. Granger,     Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92664

Jon A. Green,     Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118

Harold Grotsky,     Department of Pediatrics, Division of Infectious Diseases, Mt. Sinai School of Medicine, New York, New York 10029

Robert J. Hartzman,     Departments of Medical Genetics and Medicine, University of Wisconsin, Madison, Wisconsin

Ingegerd Hellström,     Department of Microbiology, University of Washington, Seattle, Washington 98105

Karl Erik Hellström,     Department of Pathology, University of Washington, Seattle, Washington 98105

Kurt Hirschhorn,     Department of Pediatrics, Division of Medical Genetics, Mt. Sinai School of Medicine, New York, New York 10029

Göran Holm,     Department of Immunology, The Wenner-Gren Institute, University of Stockholm, Norrtullsgatan 16, S 11345, Stockholm, Sweden

Luis Jimenez,     Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461

Jirˇí Johanovský,     Research Institute of Immunology, 108 West Pieck Street, Praha 10, Czechoslovakia

Eva Klein,     Department of Tumor Biology, Karolinska Institutet, Stockholm 60, Sweden

William P. Kolb,     Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92664

Jan Krejc,     Research Institute of Immunology, 108 West Pieck Street, Praha 10, Czechoslovakia

H. Sherwood Lawrence,     Infectious Disease and Immunology Division, Department of Medicine, New York University School of Medicine, 550 First Avenue, New York, New York 10016

Arthur S. Lebowitz,     Infectious Disease and Immunology Division, Department of Medicine, New York University School of Medicine, 550 First Avenue, New York, New York 10016

Somsak Lolekha,     Departments of Pediatrics and Microbiology, University of Illinois Medical Center, Chicago, Illinois 60612

Thomas C. Merigan,     Division of Infectious Diseases, Department of Medicine, Stanford University School of Medicine, Stanford, California 94305

Jane Nishio,     Department of Microbiology, University of Pittsburgh Medical School, Pittsburgh, Pennsylvania

Barbro Nordqvist,     Department of Dermatology, University of Lund, Lund, Sweden

Jan Pekárek,     Research Institute of Immunology, 108 West Pieck Street, Praha 10, Czechoslovakia

Hedvig Perlmann,     Department of Immunology, The Wenner-Gren Institute, University of Stockholm, Norrtullsgatan 16, S 113 45, Stockholm, Sweden

Peter Perlmann,     Department of Immunology, The Wenner-Gren Institute, University of Stockholm, Norrtullsgatan 16, S 113 45, Stockholm, Sweden

Edgar Pick,     Department of Immunology, Institute of Dermatology, University of London, London E9, England

Ross E. Rocklin,     Robert B. Brigham Hospital, 125 Parker Hill Road, Boston, Massachusetts 02115

Hans Rorsman,     Department of Dermatology, University of Lund, Lund, Sweden

Steven A. Rosenberg,     Robert B. Brigham Hospital, 125 Parker Hill Road, Boston, Massachusetts 02115

Nancy H. Ruddle,     Department of Surgery, Yale University School of Medicine, New Haven, Connecticut

Samuel B. Salvin,     Department of Microbiology, University of Pittsburgh Medical School, Pittsburgh, Pennsylvania

Richard T. Smith,     Tumor Biology Unit, Department of Pathology, University of Florida College of Medicine, Gainesville, Florida 32601

Mogens Sϕborg,     Epidemiological Department, Blegdamshospitalet, Copenhagen, Denmark

Linda Stein,     Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461

Jan Ŝvejcar,     Research Institute of Immunology, 108 West Pieck Street, Praha 10, Czechoslovakia

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Produkt-Details

• Erscheint lt. Verlag: 28.6.2014
• Sprache: englisch
• Themenwelt: Medizin / Pharmazie ► Medizinische Fachgebiete
• Themenwelt: Studium ► Querschnittsbereiche ► Infektiologie / Immunologie
• Themenwelt: Naturwissenschaften ► Biologie ► Zellbiologie
• Themenwelt: Technik ► Bauwesen
• ISBN-10: 1-4832-7759-3 / 1483277593
• ISBN-13: 978-1-4832-7759-2 / 9781483277592