Safety in Cell and Tissue Culture

G. Stacey, Alan Doyle, P. Hambleton (Herausgeber)

Buch | Softcover

244 Seiten

2012 | Softcover reprint of the original 1st ed. 1998
Springer (Verlag)
978-94-010-6061-5 (ISBN)

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It is now more than half a century since animal cells first came into regular use in the laboratory. Instances of laboratory acquired infection and con tamination of therapeutic products, derived from the use of animal cell cultures are rare. The use of animal cells, in addition to an established role in the production of vaccines and therapeutic proteins, has many new medical applications including gene therapy, tissue engineering and cell therapy. Furthermore, C;ldvances in molecular and cell biology are enabling rapid development and application of these technologies and the development of new and more sensitive methods, such as nucleic acid amplification, for the characterisation of cells and the detection of adven titious agents. However, it is clear that there is no room for complacency in this field and the recent expansion in the use of animal cells in the manufacture of medical products and the development of new biological assays for diagnostic and pharmaco-toxicological screening, underlines the need for vigilance regarding the correct and safe use of animal cells as substrates. This book is therefore very timely and should prove to be a highly valuable text, finding a wider audience beyond those with respon sibility for laboratory safety. The book guides the reader from fundamental cell biology issues and the establishment of new in vitro methods, through testing and validation of cell lines and on to issues in the use of animal cells in manufacturing processes.

1 Source Materials.- 2 Cell Biology Aspects of Safety in Cell Culture.- 3 Viral Contamination of Cell Cultures.- 4 Laboratory Practice.- 5 Planning and Design of a Cell and Tissue Culture Laboratory.- 6 Quality Control and Validation.- 7 Containment Facilities: Design, Construction and Working Practices.- 8 Scale-Up of Animal Cell Culture Systems.- 9 Production and Containment of Bioreactor Processes.- 10.1007/978-94-011-4916-7_10.- 11 Safety Aspects of Genetic Modification Procedures.- 12 International Guidelines for Safe Packaging and Transport of Biological Materials.- Appendixes to Chapter 12: A Addresses of Relevant International Organizations.- B Articles 118-120 of the Detailed Regulations of the UPU Convention.- C Countries with Import and Export Restrictions for NPBS and IPBS for National Postal Services.- D Transportation and Packaging (Numerical Order).- E Suppliers of Certified Transport Containers.- Appendix A: Classification of Microorganisms.- Appendix B: Containment Level for Cell Culture.

Zusatzinfo	IX, 244 p.
Verlagsort	Dordrecht
Sprache	englisch
Maße	155 x 235 mm
Themenwelt	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
Themenwelt	Naturwissenschaften ► Biologie ► Zoologie
ISBN-10	94-010-6061-4 / 9401060614
ISBN-13	978-94-010-6061-5 / 9789401060615
Zustand	Neuware