MicroRNAs as Tools in Biopharmaceutical Production
Seiten
2012
Springer (Verlag)
978-94-007-5127-9 (ISBN)
Springer (Verlag)
978-94-007-5127-9 (ISBN)
This book explores the uses of MicroRNAs in bioprocessing, especially in production of complex proteins in mammalian cells. Covers genomic organization, biogenesis and mode of action; miRNAs as biomarkers; manipulation of expression in cultured cells and more.
Focused manuscript on the potential use/role of miRNAs in bioprocessing, specifically the production of complex proteins in mammalian cells. With that in mind I propose a draft list of topics/chapters along the following lines: Intro on CHO/bioprocessing/engineering challenges to set scene, Genomic organization, biogenesis and mode of action, Identifying miRNA targets: Computational prediction, transcriptomics, proteomices, UTR analysis, etc., miRNA expression in Chinese Hamster Ovary cells, miRNAs as engineering targets: pathway manipulation to impact bioprocess phenotypes, miRNAs as biomarkers, Detection methods: Northern, PCR, hybridization arrays, Next Gen Seq, Manipulation of expression in cultured cells: Transient/stable disregulation, Knockout.
Focused manuscript on the potential use/role of miRNAs in bioprocessing, specifically the production of complex proteins in mammalian cells. With that in mind I propose a draft list of topics/chapters along the following lines: Intro on CHO/bioprocessing/engineering challenges to set scene, Genomic organization, biogenesis and mode of action, Identifying miRNA targets: Computational prediction, transcriptomics, proteomices, UTR analysis, etc., miRNA expression in Chinese Hamster Ovary cells, miRNAs as engineering targets: pathway manipulation to impact bioprocess phenotypes, miRNAs as biomarkers, Detection methods: Northern, PCR, hybridization arrays, Next Gen Seq, Manipulation of expression in cultured cells: Transient/stable disregulation, Knockout.
Preface.-1 A general introduction to microRNAs, their investigation and exploitation in CHO cell lines.-2 Biogenesis of Mammalian miRNA.-3 Target prediction algorithms and bioinformatics resources for miRNA studies.-4 The CHO miRNA transcriptome.-5 MicroRNAs as engineering targets - pathway manipulation to impact bioprocess phenotypes.-6 MicroRNAs for Enhancement of CHO Cell Proliferation and Stability Insights from Neuroblastoma Studies.-7 MicroRNA Detection Methods for Mammalian Cell Lines and Their Applications in Therapeutic Protein Production.-8 Manipulating Levels of Specific microRNAs in Mammalian Cells.- Index.
| Zusatzinfo | X, 126 p. |
|---|---|
| Verlagsort | Dordrecht |
| Sprache | englisch |
| Maße | 155 x 235 mm |
| Themenwelt | Medizin / Pharmazie ► Pharmazie |
| Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
| Naturwissenschaften ► Biologie ► Zoologie | |
| Naturwissenschaften ► Chemie ► Technische Chemie | |
| Technik ► Umwelttechnik / Biotechnologie | |
| Schlagworte | Bioprocessing • Chinese Hamster Ovary cells • microRNA • Recombinant Protein Production |
| ISBN-10 | 94-007-5127-3 / 9400751273 |
| ISBN-13 | 978-94-007-5127-9 / 9789400751279 |
| Zustand | Neuware |
| Informationen gemäß Produktsicherheitsverordnung (GPSR) | |
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