Laboratory Techniques in Membrane Biophysics

An Introductory Course

Heinz Passow, Robert Stämpfli (Herausgeber)

Buch | Softcover

VIII, 201 Seiten

1969
Springer Berlin (Verlag)
978-3-540-04592-2 (ISBN)

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The present manual contains a collection of laboratory instructions used during an international training course on membrane biophysics which was held at Homburg in the fall of 1966. The selection of the topics dealt with in the various chapters depended on the scientific interest of the available teachers and on the availability of the necessary equipment in our laboratories. Thus, the material included in this volume does not add up to a systematic course in membrane biophysics. Instead it represents a more fortuitous collection of laboratory problems. In addition, some authors place more emphasis on teaching the more technical aspects of a method whereas others are primarily concerned with the demonstra tion of a significant biological phenomenon. Nevertheless, in spite of such differences of emphasis and a somewhat haphazard choice of a few methods and phenomena among many others of similar importance, it was felt that the publication of the material is desirable. Since no other laboratory manual exists so far, the present laboratory problems which were tested in actual practice may serve as a useful basis for the shaping of further training courses or for laboratory courses for graduate students in biophysics and physiology. Our thanks are due to the authors and the publisher who were patient and kind enough to cooperate with the editors during the long period between the end of the course and the appearance of the book.

Measurement of Hydraulic Conductivity and Reflexion Coefficients of a Plant Cell Membrane by means of Transcellular Osmosis.- Flux Measurements in Erythrocytes.- Ion Permeability of Erythrocyte Ghosts.- Countertransport in Human Red Blood Cells.- Kinetics of Cation Transport in Yeast.- Bacterial Permeases.- The Exchange of 22Na between Frog Sartorius Muscle and the Bathing Medium.- Determination of Intracellular Ionic Concentrations and Activities.- Experiments on Na Transport of Frog Skin Epithelium.- Micropuncture and Microanalysis in Kidney Physiology.- Oscillatory Phenomena in a Porous, Fixed Charge Membrane.- Bimolecular Lipid Membranes: Techniques of Formation, Study of Electrical Properties, and Induction of Ionic Gating Phenomena.- Dissection of Single Nerve Fibres and Measurement of Membrane Potential Changes of Ranvier Nodes by means of the Double Air Gap Method.- Ionic Currents in the Myelinated Nerve Fibre.- A New Voltage Clamp Method.- Electrical Activity of Synapses. The Crayfish Neuromuscular Junction.- Author Index.

Erscheint lt. Verlag	1.1.1969
Zusatzinfo	VIII, 201 p.
Verlagsort	Berlin
Sprache	englisch
Maße	152 x 229 mm
Gewicht	360 g
Themenwelt	Studium ► 1. Studienabschnitt (Vorklinik) ► Physiologie
	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Physik / Astronomie ► Angewandte Physik
Schlagworte	Biophysics • blood cell • erythrocyte • Laboratoriumstechnik • Lipid • Membran • Membrane • Physiology • Synapse
ISBN-10	3-540-04592-9 / 3540045929
ISBN-13	978-3-540-04592-2 / 9783540045922
Zustand	Neuware