Molecular Microbiology

Diagnostic Principles and Practice

David H Persing (Autor)

Buch | Hardcover

960 Seiten

2010 | 2nd New edition
American Society for Microbiology (Verlag)
9781555814977 (ISBN)

Infos

provides complete coverage of the principles and applications of molecular microbiology as they pertain to infectious diseases; summarizes the basic scientific theory underlying molecular diagnostics; reviews proven applications of molecular diagnostic techniques for the diagnosis of infectious diseases.

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The latest molecular diagnostic techniques presented in one comprehensive volume.




Offers a broad range of practical advice, such as determining the appropriate type and quantity of a specimen, releasing and concentrating the targets, and eliminating inhibitors.
Examines techniques to detect individual pathogens and the growing trend toward a systems approach for diagnosing infectious diseases, with chapters covering gastrointestinal infections, sepsis, meningitis, and encephalitis.
Features updated information on molecular tests, laboratory operations, molecular typing, and new technologies for nucleic acid detection; plus, the latest information on quality control, quality assurance, and laboratory design to help ensure a safe, efficient molecular laboratory.








This title is published by the American Society of Microbiology Press and distributed by Taylor and Francis in rest of world territories.

Inhalt

Table of Contents


DIAGNOSTIC PRINCIPLES



I. Molecular Hybridization for Microbial Identification

1. Applications of Fluorescence In Situ Hybridization in Diagnostic Microbiology

Stefan Juretschko and Thomas R. Fritsche

2. DNA Probes for Culture Confirmation and Direct Detection of Bacterial and Fungal Infections: a Review of Current Technologies and Assays

Julie Kingery and Karen C. Carroll



II. Nucleid Acid Amplification Methods

3. In Vitro Nucleic Acid Amplification Techniques

Vivekanand Datta and Randall T. Hayden

4. Real Time PCR and Melting Analysis

Carl T. Wittwer and Noriko Kusukawa

5. Quantitative Molecular Methods

Donna M. Wolk and Randall T. Hayden

6. Use of Commercial Amplification Tests in the Clinical Microbiology Laboratory: Test Selection and Quality Assurance

Gerri S. Hall, Mark Katanik, Marion Tuohy, and Mary Sholtis



III. Laboratory Operations

7. Nucleic Acid Isolation: Overview of Sample Preparation Methods

Charles E. Hill

8. Laboratory Design and Operations

P. Shawn Mitchell, Jeffrey J. Germer, and Joseph D. C. Yao



IV. Molecular Typing Methods

9. Molecular Phylogenetic Analysis

Les Dethlefsen, Paul W. Lepp, and David A. Relman

10. Pulsed-Field Gel Electrophoresis: Laboratory and Epidemiologic Considerations for Interpretation of Data

Richard V. Goering, Efrain M. Ribot, and Peter Gerner-Smidt

11. Strain Typing Using Multiple Loci "Variable Number of Tandem Repeat" Analysis and Genetic Element CRISPR

Christine Pourcel and Gilles Vergnaud

12. Repetitive Sequence-Based PCR Typing of Bacteria and Fungi

Stacie R. Frye and Mimi Healy

13. Molecular Typing Methods for Analysis of Extraintestinal Pathogenic Escherichia coli

James R. Johnson



V. Detection Methodology

14. Fluorescence Resonance Energy Transfer

J. R. Uhl, Y.-W. Tang, and F. R. Cockerill III

15. Detection of Pathogenic Organisms with Multicolor Molecular Beacons

Fann Wu, Phyllis Della-Latta, Sanjay Tyagi, and Fred Russell Kramer

16. Microwell Plate Detection Systems for Amplicon Detection and Characterization

Jiang Fan and Michael J. Loeffelholz

17. Detection Methodology: Pyrosequencing

Liying Yan and Robert Slinger

18. Solid- and Liquid-Phase Array Technologies

Melissa B. Miller



VI. New Technologies

19. Next Generation DNA Sequencing and Microbiology

Russell Higuchi, Ulf Gyllensten and David H. Persing

20. Raman Spectroscopy for Bacterial Strain Typing

Diana Willemse-Erix, Alex van Belkum, and Kees Maquelin

21. Multiplex PCR Product Detection and Discrimination

Steven Zink, Nick M. Cirino, and Christina Egan

22. Detection of Emerging Antimicrobial Resistance by Use of the Ibis T5000 Universal Biosensor

David J. Ecker, Rangarajan Sampath, Christian Massire, Lawrence B. Blyn, Mark W. Eshoo, Thomas A. Hall, and Steven Hofstadler



DIAGNOSTIC APPLICATIONS



VII. Molecular Detection of Bacterial Organisms

23. Update on the Detection and Characterization of Bacterial Pathogens by Nucleic Acid Amplification

K. Loens, H. Goossens, and M. Ieven

24. Molecular Detection of Chlamydia trachomatis and Neisseria gonorrhoeae

Jens K. Moller, Bjoern Herrmann, Jorgen Skov Jensen, and Henrik Westh

25. PCR Detection of Haemophilus ducreyi, Treponema pallidum and Mycoplasma genitalium

Patricia A. Totten, Lisa E. Manhart, and Arturo Centurion-Lara

26. Molecular Detection and Characterization of Mycobacterium tuberculosis

Betty A. Forbes

27. Sequence-Based Identification and Characterization of Mycobacteria

Nancy L. Wengenack and Leslie Hall

28. Molecular Detection of Group B Streptococcus

Elizabeth M. Marlowe and Preeti Pancholi

29. Molecular Detection and Identification of Methicillin-Resistant Staphylococcus aureus

Willem van Leeuwen and Alex van Belkum

30. Identification of Bacteria by DNA Target Sequencing in a Clinical Microbiology Laboratory

Rosemary C. She, Keith E. Simmon, and Cathy A. Petti

31. Broad-Range PCR for Detection and Identification of Bacteria

Matthias Maiwald

32. Detection of Antimicrobial Resistance Genes and Mutations Associated with Antimicrobial Resistance in Bacteria

Fred C. Tenover and J. Kamile Rasheed

33. Detection of Agents Associated with Bioterrorism

Paul Keim, Victor Waddell, and David M. Engelthaler



VIII. Molecular Detection and Characterization of Viruses

34. Diagnostic Molecular Virology: Current Practice and Future Trends

Frederick S. Nolte

35. Molecular Detection and Characterization of HIV-1

Angela M. Caliendo and Colleen S. Kraft

36. Molecular Detection and Characterization of Hepatitis C Virus

Michael S. Forman and Alexandra Valsamakis

37. Molecular Detection and Characterization of Hepatitis B Virus

David R. Hillyard

38. Molecular Detection of Human Papillomaviruses

Denise I. Quigley and Elizabeth R. Unger

39. Molecular Detection of Respiratory Viruses

Richard S. Buller and Max Q. Arens

40. Viral Infections in Transplant Recipients

Robin Patel and Frederick S. Nolte

41. New Virus Discovery in the 21st Century

Stacy Finkbeiner and David Wang



IX. Detection and Characterization of Fungal Pathogens

42. Molecular Detection and Characterization of Fungal Pathogens

Stephane Bretagne

43. Sequence-Based Fungal Identification and Classification

Jianli Dong, Michael J. Loeffelholz, and Michael R. McGinnis

44. Molecular Detection of Antifungal Resistance

Thomas D. Edlind



X. Detection and Characterization of Parasitic Protozoa

45. Overview of the Development, Utility, and Future of Molecular Diagnostics for Parasitic Diseases

Bobbi Pritt and Jon Rosenblatt

46. Molecular Approaches for Diagnosis of Malaria and Characterization of Genetic Markers of Drug Resistance

Laura K. Erdman, Michael Hawkes, and Kevin C. Kain

47. Molecular Approaches for Diagnosis of Chagas' Disease and Genotyping of Trypanosoma cruzi

Michal Svoboda, Myrna Virreira, Carine Truyens, Faustino Torrico, and Yves Carlier



XI. Systems Microbiology

48. Molecular Diagnosis of Gastrointestinal Infections

Benjamin A. Pinsky and Niaz Banaei

49. Molecular Approaches to the Diagnosis of Sepsis

Paul H. M. Savelkoul and Remco P. H. Peters

50. Molecular Approaches to the Diagnosis of Meningitis and Encephalitis

Karen C. Bloch and Yi-Wei Tang



XII. The Pathogen/Host Interface

51. Genomics and DNA Variation: Determinants of Susceptibility and Outcomes in Microbial Diseases

Chiea-Chuen Khor, Stephen J. Chapman, and Adrian V. S. Hill

52. Genetically Polymorphic Cytochrome P450s and Transporters and Personalized Antimicrobial Chemotherapy

Hong-Guang Xie

53. Host Genomic Profiling in Human Immunodeficiency Virus Infection

Kevin V. Shianna and Amalio Telenti

54. Innate Immunity and Host Defense against Microbial Infection

Markus Schnare and Salman Qureshi



XIII. Laboratory Standardization, Proficiency-Testing Programs, Quality-Control Standards, and Monitoring

55. Molecular Method Verification

Donna M. Wolk and Elizabeth M. Marlowe

56. Molecular Test Validation, Monitoring, and Quality Control

Matthew J. Bankowski

57. External Quality Assessment and Proficiency Testing in Diagnostic Molecular Microbiology

Michael J. Mitchell

58. Laboratory Controls and Standards

Maurice Exner

Produkt-Details

• Verlagsort: Washington DC
• Sprache: englisch
• Gewicht: 2572 g
• Einbandart: gebunden
• Themenwelt: Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin
• ISBN-13: 9781555814977 / 9781555814977
• Zustand: Neuware