Studies on the Confocal Laser Microscope (eBook)

Abdallah Mohamed Hamed (Autor)

eBook Download: PDF

2025 | 1. Auflage
XIII, 143 Seiten
Springer-Verlag
978-3-031-87275-4 (ISBN)

Infos

This book provides a thorough exploration of various modulated apertures and their impact on improving microscope resolution, with a focus on confocal scanning laser microscopy (CSLM). Over the course of eleven chapters, it looks at both the theoretical aspects and practical applications of different aperture shapes.

Chapters 1 and 2 review apertures with linear, quadratic, and concentric black-and-white (B/W) zones, along with linear-quadratic and polynomial designs. Additionally, apertures with Hamming, Cauchy, rectangular, and hexagonal shapes are analyzed for their potential to enhance imaging performance.

Chapter 3 presents the computation of coherent transfer functions (CTFs) for selected modulated apertures, offering insights into their influence on imaging quality. Chapter 4 focuses on confocal microscopes, exploring how these apertures affect the imaging of microscopic objects.

A theoretical study of coherent non-scanned laser microscopes (CNSM) is covered in Chapter 5. Chapter 6 addresses the computation of lateral and axial point spread functions (PSFs) in confocal imaging systems that use binary amplitude masks, while Chapter 7 investigates the effects of misalignment errors combined with wavefront aberrations in systems using linear and quadratic apertures.

In Chapter 8, diffraction intensity is calculated for a confocal microscope with a laterally displaced truncated Gaussian aperture, extending the principles of Marechal microscopy to confocal scanning microscopy. Chapters 9 and 10 examine spatial coherence in confocal optical systems, particularly in the context of quadratic and concentric B/W apertures.

The book concludes in Chapter 11 with an application of cardiac apertures in CSLM, demonstrating their use in processing cardiac images. This work serves as a valuable reference for researchers and professionals interested in advancing microscope resolution through innovative aperture design and analysis.

Produkt-Details

• Erscheint lt. Verlag: 29.3.2025
• Reihe/Serie: SpringerBriefs in Applied Sciences and Technology
• Zusatzinfo: XIII, 143 p. 91 illus., 42 illus. in color.
• Sprache: englisch
• Themenwelt: Mathematik / Informatik ► Informatik ► Grafik / Design
• Themenwelt: Naturwissenschaften ► Physik / Astronomie ► Optik
• Schlagworte: Aberrations and Excentration Errors in Confocal Microscope • Black and White Aperture • Cauchy and Hamming Apertures • Confocal laser scanning microscope • Linear and Quadratic Apertures • Polynomial Aperture • Rectangular and Hexagonal Apertures • Spatial Coherence in Confocal Microscopy
• ISBN-10: 3-031-87275-4 / 3031872754
• ISBN-13: 978-3-031-87275-4 / 9783031872754