Plant Circular RNAs

Methods and Protocols

Buch | Softcover

196 Seiten

2022 | 2021 ed.
Springer-Verlag New York Inc.
978-1-0716-1647-5 (ISBN)

Artikel merken

This volume presents readers with up-to-date protocols, bioinformatics toolkits, and reference material for understanding circRNAs in plants. The chapters in this book summarize the concepts and techniques for prediction/identification, validation, and analysis of plant circRNAs and their regulatory targets. Some of the topics covered are procedures for circRNA identification and characterization; strategies for circRNA generation; next generation sequencing (NGS) technologies and circRNAs’ research; and bioinformatics tools and pipelines for the analysis of circRNA-miRNA-mRNA regulatory networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Circular RNAs: Methods and Protocols is an important reference for any researcher looking to explore and apply the latest developments made in this field.

Identification and Characterization of Plant Circular RNAs.- Experimental Strategies for Studying the Function of Plant circRNAs.- Generation of Transgenic Rice Expressing circRNA and its Functional Characterization.- Identification of Circular RNAs by Multiple Displacement Amplification and their Involvement in Plant Development.- Identification of Intronic Lariat-Derived Circular RNAs in Arabidopsis by RNA Deep Sequencing.- Identification and Functional Characterization of Viroid Circular RNAs.- Circular RNA Databases.- NGS Methodologies and Computational Algorithms for the Prediction and Analysis of Plant Circular RNAs.- Computational Analysis of Transposable Elements and circRNAs in Plants.- Constructing circRNA-miRNA-mRNA Regulatory Networks by using GreenCircRNA Database.- Methods for Predicting circRNA-miRNA-mRNA Regulatory Networks: GreenCircRNA and PlantCircNet Databases as Study Cases.

Erscheinungsdatum	04.07.2022
Reihe/Serie	Methods in Molecular Biology ; 2362
Zusatzinfo	43 Illustrations, black and white; X, 196 p. 43 illus.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Mathematik / Informatik ► Informatik ► Theorie / Studium
	Informatik ► Weitere Themen ► Bioinformatik
	Naturwissenschaften ► Biologie ► Botanik
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
Schlagworte	circular RNAs (circRNAs) • comparative genomics • Quantitative real-time PCR (qRT-PCR) • RNA immunoprecipitation • Sequencing Data
ISBN-10	1-0716-1647-1 / 1071616471
ISBN-13	978-1-0716-1647-5 / 9781071616475
Zustand	Neuware