Metabolic Flux Analysis in Eukaryotic Cells

Methods and Protocols

Buch | Softcover

370 Seiten

2021 | 2020 ed.
Springer-Verlag New York Inc.
978-1-0716-0161-7 (ISBN)

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This volume explores the latest metabolic flux analysis (MFA) techniques that cover the analysis of cellular, organ level, and whole-body metabolism. The chapters in this book discuss topics such as deutrium tracing; isotopologue fractions using GC-TOF; non-targeted mass isotopolome analysis; large-scale profiling of cellular metabolic activities using deep 13C labeling medium; metastases in mice; SWATH; Exo-MFA; metabolic flux from time-course metabolomics; and thermodynamic approaches in flux analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Metabolic Flux Analysis in Eukaryotic Cells: Methods and Protocols is a valuable resourcefor both experts in MFA techniques and researchers getting involved in the role of quantitative studies to uncover the dysregulated pathways in human diseases.

Determination of Isotopologue and Tandem Mass Isotopologue Fractions using Gas Chromatography Chemical Ionization Time of Flight Mass Spectrometry: Methodology and Uncertainty of Measurement.- Non-Targeted Mass Isotopolome Analysis using Stable Isotope Patterns to Identify Metabolic Changes.- Liquid Chromatography Methods for Separation of Polar and Charged Intracellular Metabolites for 13C Meabolic Flux Analysis.- Deuterium Tracing to Interrogate Compartment-Specific NAD(p)H Metabolism in Cultured Mammalian Cells.- Large-Scale Profiling of Cellular Metabolic Activities using Deep 13C Labeling Medium.- Analyzing the Metabolism of Metastases in Mice.- Robusy Analytical methods for the Accurate Quantification of the Total Biomass Composition of Mammalian Cells.- Quantifying Nitric Oxide Flux Distributions.- SWATH: A Data-Independent Tandem Mass Spectrometry Method to Quantify 13C Enrichment in Cellular Metabolites and Fragments.- Quantifying Metabolic Transfer Mediated by Extracellular Vesicles using Exo-MFA: An Integrated Empirical and COmputational Platform.- Comparative Metabolic Network Flux Analysis to Identify Differences in Cellular Metabolism.- Software Supporting a Workflow of Quantitative Dynamic Flux Maps Estimation in Central Metabolism from SIRM Experimental Data.- Inferring Metabolic Flux from Time-Course Metabolomics.- Using Metabolic Network Reconstructions to Predict Drug Targets and Off-Target Effects.- Single-Cell Digital Twins for Cancer Preclinical Investigation.- Supply and Demand Analysis of Autophagy.- Thermodynamic Approaches in Flux Analysis.

Erscheinungsdatum	18.01.2021
Reihe/Serie	Methods in Molecular Biology ; 2088
Zusatzinfo	64 Illustrations, color; 23 Illustrations, black and white; XIII, 370 p. 87 illus., 64 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Mathematik / Informatik ► Informatik ► Theorie / Studium
	Informatik ► Weitere Themen ► Bioinformatik
	Naturwissenschaften ► Biologie ► Zellbiologie
	Technik ► Umwelttechnik / Biotechnologie
Schlagworte	Dynamic stable-isotope modeling • Exosome-mediated metabolic flux analysis • Lipid metabolism • pSIRM • transcriptional regulatory networks
ISBN-10	1-0716-0161-X / 107160161X
ISBN-13	978-1-0716-0161-7 / 9781071601617
Zustand	Neuware