DNA Repair Enzymes: Cell, Molecular, and Chemical Biology

Buch | Hardcover

478 Seiten

2017
Academic Press Inc (Verlag)
978-0-12-811846-7 (ISBN)

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DNA Repair Enzymes, Part A, Volume 591 is the latest volume in the Methods in Enzymology series and the first part of a thematic that focuses on DNA repair enzymes. Topics in this new release include chapters on the Optimization of Native and Formaldehyde iPOND Techniques for Use in Suspension Cells, the Proteomic Analyses of the Eukaryotic Replication Machinery, DNA Fiber Analysis: Mind the Gap!, Comet-FISH for Ultrasensitive Strand-Specific Detection of DNA Damage in Single Cells, Examining DNA Double-Strand Break Repair in a Cell Cycle-Dependent Manner, Base Excision Repair Variants in Cancer, and Fluorescence-Based Reporters for Detection of Mutagenesis in E. coli.

Dr. Eichman is a Professor of Biological Sciences and Biochemistry at Vanderbilt University, where his laboratory investigates the structural mechanisms of protein machines involved in maintenance of genome integrity. Professor Eichman was initially trained as a synthetic organic chemist at the University of Mississippi (B.S., Chemistry, 1993). He received his Ph.D. in Biochemistry and Biophysics in 2000 from Oregon State University, where he used X-ray crystallography to study the effects of crosslinking agents on DNA structure and determined the landmark structure of the Holliday junction, the four-stranded DNA intermediate formed during genetic recombination. As an NIH postdoctoral fellow from 2000-2004 with Tom Ellenberger at Harvard Medical School, Eichman studied the structural enzymology of DNA repair and replication proteins. Current projects in the Eichman lab focus on base excision repair of DNA alkylation damage and restart of stalled replication forks during the DNA damage response. Dr. Eichman holds the 2009 Young Investigator Award from the Sigma Xi Scientific Research Society, the Vanderbilt Chancellor’s Award for Research, two Vanderbilt-Ingram Cancer Center Impact Awards, and in 2013 became a member of the Faculty of 1000. Eichman teaches introductory and advanced undergraduate biochemistry and serves as the co-Director of the Vanderbilt Undergraduate Program in Biochemistry and Chemical Biology.

1. Optimization of Native and Formaldehyde iPOND Techniques for Use in Suspension Cells
Nathaniel E. Wiest and Alan E. Tomkinson
2. Proteomic Analyses of the Eukaryotic Replication Machinery
David Cortez
3. DNA Fiber Analysis: Mind the Gap!
Annabel Quinet, Denisse Carvajal-Maldonado, Delphine Lemacon and Alessandro Vindigni
4. Comet-FISH for Ultrasensitive Strand-Specific Detection of DNA Damage in Single Cells
Manas Mondal and Jia Guo
5. Examining DNA Double-Strand Break Repair in a Cell Cycle-Dependent Manner
Janapriya Saha, Shih-Ya Wang and Anthony J. Davis
6. Base Excision Repair Variants in Cancer
Carolyn G. Marsden, Julie A. Dragon, Susan S. Wallace and Joann B. Sweasy
7. Fluorescence-Based Reporters for Detection of Mutagenesis in E. coli
Melissa Standley, Jennifer Allen, Layla Cervantes, Joshua Lilly and Manel Camps
8. Sequencing DNA for the Oxidatively Modified Base 8-Oxo-7,8-Dihydroguanine
Aaron M. Fleming, Yun Ding and Cynthia J. Burrows
9. Xenopus laevis as Model System to Study DNA Damage Response and Replication Fork Stability
Vincenzo Sannino, Federica Pezzimenti, Stefania Bertora and Vincenzo Costanzo
10. Ensemble and Single-Molecule Analysis of NHEJ in Frog Egg Extracts
Thomas Graham, Johannes C. Walter and Joseph J. Loparo
11. Analysis of Structure-Selective Endonuclease Activities From Yeast and Human Extracts
Joao Matos and Stephen C. West
12. Strategies and Methods of Transcription-Coupled Repair Studies In Vitro and In Vivo
Vitaly Epshtein, Venu Kamarthapu and Evgeny Nudler
13. Reconstituted System for the Examination of Repair DNA Synthesis in Homologous Recombination
Youngho Kwon, James M. Daley and Patrick Sung
14. Current and Emerging Assays for Studying the Primer Synthesis Activities of DNA Primases
Thomas A. Guilliam and Aidan J. Doherty
15. Electrical Probes of DNA-Binding Proteins
Jacqueline K. Barton, Phillip L. Bartels, Yingxin Deng and Elizabeth O’Brien
16. Preparation of Stable Nitrogen Mustard DNA Interstrand Cross-Link Analogs for Biochemical and Cell Biological Studies
Alejandra Castaño, Upasana Roy and Orlando D. Schärer

Erscheinungsdatum	06.07.2017
Reihe/Serie	Methods in Enzymology
Verlagsort	San Diego
Sprache	englisch
Maße	152 x 229 mm
Gewicht	930 g
Themenwelt	Informatik ► Weitere Themen ► Bioinformatik
	Medizin / Pharmazie ► Medizinische Fachgebiete
	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Zellbiologie
ISBN-10	0-12-811846-6 / 0128118466
ISBN-13	978-0-12-811846-7 / 9780128118467
Zustand	Neuware