Functions of Transcription Factor Alpha-Pal/NRF-1 in Neurons

Alpha-Pal/NRF-1 regulates IAP/CD47 Expression and Neurite Outgrowth in Neurons

Wen-Teng Chang, A-Min Huang (Autoren)

Buch | Softcover

92 Seiten

2008
VDM Verlag Dr. Müller e.K.
978-3-8364-9683-4 (ISBN)

Titel ist leider vergriffen;
keine Neuauflage

Artikel merken

This book adopts modern molecular biology techniques to provide evidence demonstrating the transcription factor alpha-Pal/NRF-1 is a critical regulator of the human IAP/CD47 gene.This book provides evidence to demonstrate a novel function of alpha-Pal/NRF-1 in regulation of neurite outgrowth in neurons and this function is mediated by its downstream gene IAP/CD47.This book describes practical tips for promoter studies and protein functions in neurons, such as RNA isolation, reverse-transcription-polymerase chain reaction, site-directed mutagenesis, deletion constructs, electrophoretic mobility shift and supershift assays, luciferase assays, isolation of stable cell lines, primary neuronal cultures, transient transfection, immunofluorescence microscopy, and measurements of neurite outgrowth. Therefore, this is a reference book for molecular neuroscientists to study neuronal genes and functions.

Chang, Wen-Teng Dr Wen-Teng Chang is Assistant Professor in the Department of Biological Science and Technology at Chung Hwa University of Medical Technology, Tainan, Taiwan. Huang, A-Min Dr A-Min Huang is Associate Professor in Department of Physiology and Institute of Basic Medical Sciences at National Cheng Kung University, Tainan, Taiwan.

Sprache	englisch
Maße	150 x 6 mm
Gewicht	140 g
Themenwelt	Naturwissenschaften ► Chemie ► Allgemeines / Lexika
ISBN-10	3-8364-9683-6 / 3836496836
ISBN-13	978-3-8364-9683-4 / 9783836496834
Zustand	Neuware