Molecular Diagnostics in Cytopathology

A Practical Handbook for the Practicing Pathologist

Sinchita Roy-Chowdhuri, Paul A. VanderLaan, John M. Stewart, Gilda da Cunha Santos (Herausgeber)

Buch | Softcover

XV, 534 Seiten

2018 | 1st ed. 2019
Springer International Publishing (Verlag)
978-3-319-97396-8 (ISBN)

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Molecular diagnostics are increasingly used to help guide targeted therapy in solid organ tumors and hematologic malignancies. A large proportion of molecular testing is performed on limited-volume samples obtained via minimally invasive techniques, such as fine needle aspiration. Increasingly, cytopathologists play an essential role in this process, both in the triage of specimens during rapid on-site evaluation and in the evaluation of archival samples to determine suitability for ancillary testing. Therefore, it is imperative that practicing cytopathologists stay abreast of up-to-date diagnostic, prognostic, and predictive ancillary tests that can be used on limited cytologic material. This is a challenge since the landscape of known genomic alterations is constantly evolving and the subsequent set of testing options is ever expanding.
The proposed text will provide a user-friendly quick-reference handbook to serve as a useful resource for practicing pathologists and laboratory personnel dealing with, and interested in, this evolving field of molecular cytopathology. Essential components to be presented include: 1) pre-analytic factors that affect sample selection and evaluation; 2) specimen preparation to maximize confidence in results; 3) interpretation of results; 4) potential limitations; and 5) workflow algorithms. In addition, specific disease specific molecular testing details will be outlined to provide the reader with resources for quick reference. All chapters will be written by experts in their fields and will include the most up-to-date scientific and clinical information.
Molecular Diagnostics in Cytopathology will be of value to Cytopathologists, Cytotechnologists, Cytotechnology students, Cytopathology fellows, Surgical pathologists, Pathology residents and fellows, Molecular Pathologists, Molecular pathology fellows, Molecular technologists, as well as Translational researchers with an interest in molecular cytopathology.

Sinchita Roy-ChowdhuriThe University of Texas MD Anderson Cancer CenterHouston, TXUSA Paul A. VanderLaan Beth Israel Deaconess Medical CenterHarvard Medical SchoolBoston, MAUSA John M. StewartThe University of Texas MD Anderson Cancer CenterHouston, TXUSA Gilda da Cunha SantosSunnybrook Health Science CenterToronto, OntarioCanada

Introduction: Overview of Current Molecular Diagnostic Testing on Cytology Samples.- The Cytology Specimen and Preparations: Advantages and Limitations.- Sample Acquisition and Test Requisition.- Pre-Analytic Workflow and Specimen Evaluation.- DNA-Based Sequencing Assays.- RNA-Based Assays.- FISH Testing of Cytology Specimens: Pre-Analytic, Analytic and Post-Analytic Considerations.- Molecular Cytopathology Correlations: Interpretation of Molecular Diagnostic Results.- Human Papillomavirus (HPV) Testing of Head and Neck Cancers.- Human Papillomavirus (HPV) Testing on Cervical Cytology Specimens.- Molecular Diagnostics in Lung Cytology.- Molecular Diagnostics in Thyroid Cytology.- Molecular Diagnostics in Breast Cytology.- Molecular Diagnostics in Salivary Gland Cytology.- Molecular Diagnostics in Pancreatic and Biliary Cytology.- Fluorescence In Situ Hybridization (FISH) Testing in Urinary Tract Cytology.- Molecular Diagnostics in Hematological Malignancies.- Molecular Diagnostics in Bone and Soft Tissue Tumors.- Molecular Diagnostics in Pediatric Cytopathology.- Molecular Cytopathology: Final Thoughts and Future Directions.

Erscheinungsdatum	10.11.2018
Zusatzinfo	XV, 534 p. 93 illus., 91 illus. in color.
Verlagsort	Cham
Sprache	englisch
Maße	127 x 203 mm
Gewicht	592 g
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
Themenwelt	Studium ► 2. Studienabschnitt (Klinik) ► Pathologie
Schlagworte	cytology specimen • FISH assays • Human papillomavirus • Laboratory processing • RT-PCR assays • Solid organ malignancies
ISBN-10	3-319-97396-7 / 3319973967
ISBN-13	978-3-319-97396-8 / 9783319973968
Zustand	Neuware